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CoV–2 Spike Impairs DNA Damage Repair – Inhibits V(D)J Recombination in Vitro

mdpi.com

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Re: CoV–2 Spike Impairs DNA Damage Repair – Inhibits V(D)J Recombination in Vitro

#81

Post by AstralStorm »

lend000 wrote: post #68

This is one of the more accessible and straightforward microbiology papers I've read. There's no fluff; it tells you how to reproduce and where to buy the materials, and it seems to balance terms in the jargon-heavy field with short explanations, requiring minimal googling to get an overview if you are topically familiar cell biology and chemistry (not to mention VDJ recombination, a fascinating bio-algorithm which s…

The implication being that unmodified spike protein and protein producing vaccines could be less effective than some modified ones and also cause a temporary dip in immunity due to locking out some naive B cells, is that correct?

Re: CoV–2 Spike Impairs DNA Damage Repair – Inhibits V(D)J Recombination in Vitro

#82

Post by LurkingPenguin »

GhettoComputers wrote: post #33
LurkingPenguin wrote: post #23

Earlier quoted context omitted.

> I agree, but the mRNA vaccines factually aren't long lasting. Get your booster if you haven't yet, its important since the protection is about half in under half a year. This is misinformation. From your link: > Vaccine effectiveness against hospital admissions for infections with the delta variant for all ages was high overall (93% [95% CI 84–96]) up to 6 months. A post regarding a possible "issue" with the vaccin…

"Up to" 6 months isn't long lasting, thats salesmen speak, and the average again was 53% after 4 months. >vaccine effectiveness against infections of the delta variant was high during the first month after full vaccination (93% [95% CI 85–97]) but declined to 53% [39–65] after 4 months. >Reduction in vaccine effectiveness against SARS-CoV-2 infections over time is probably primarily due to waning immunity with time r…

I pointed out that the VE against hospitalization remains high (>90%) at 6 months and you continue to highlight VE against infection. These are not the same endpoints.

> People don't want to get infected so they don't get long covid.

Notwithstanding the fact there's some evidence the vaccines don't protect as well against long COVID as previously suggested[1], if this is the case and large numbers of people are eager to boost every 4-6 months out of fear they might get long COVID if they don't, you don't need to remind everyone to "get your booster!"

We should be able to have discussions about the virus and vaccines without gratuitous PSAs.

[1] https://www.medrxiv.org/content/10.1101/2021.10.26.21265508v...

Re: CoV–2 Spike Impairs DNA Damage Repair – Inhibits V(D)J Recombination in Vitro

#83

Post by pas »

yarg wrote: post #64
jaspergilley wrote: post #32

User @shusaku refers to MDPI as a "garbage journal". If true, this info should play a bigger part in the discussion here than it currently is

Seems to be a moot point? https://pubmed.ncbi.nlm.nih.gov/34696485/

why? it still links to MDPI.

Re: CoV–2 Spike Impairs DNA Damage Repair – Inhibits V(D)J Recombination in Vitro

#84

Post by pas »

ra wrote: post #22

Please can someone who is an expert in this field explain this in simple terms for us lay people?

not an expert, but you might find this interesting: https://www.youtube.com/watch?v=9E_UxnC_L2o

VDJ recombination is a "random number generator" that helps diversify receptors. (literally it shuffles the a very specific part of the genome of the developing immune cell), which leads to an almost surely new unique receptor that can match previously "undetected pathogens". this makes it possible for the adaptive immune system to have a higher efficiency. (of course if it matches something from the host, it's an autoimmune problem. but there's a step during immune cell development where these misbehaving cells are detected and destroyed, which generally works very well.)

Re: CoV–2 Spike Impairs DNA Damage Repair – Inhibits V(D)J Recombination in Vitro

#85

Post by koolba »

revolvingocelot wrote: post #47
koolba wrote: post #38

Earlier quoted context omitted.

> Searching for a means by which the vaccine might meaningfully damage your adaptive immune system is ill-advised. Frankly, we've got terrifying amounts of information about the deleterious effects of catching and surviving serious COVID, and administration of a vaccine tends to uncontroversially downgrade the seriousness of the COVID infection that you will almost assuredly get eventually. Thus, even if the vaccine…

>Explicitly saying nobody should look into a potential negative side effect of vaccines because they might actually find something is down right scary. You didn't read the article. Or what I wrote about it. The vaccine can't do any worse than the virus, and this study, that does look into a potential negative side effect , found that the virus does indeed do worse! Scaremongering while putting words into someone else…

> You didn't read the article. Or what I wrote about it.

I literally quoted your words. Are you suggesting I randomly selected text and copy/pasted it without reading it?

> The vaccine can't do any worse than the virus, and this study, that does look into a potential negative side effect, found that the virus does indeed do worse!

Nothing in what I said implied that the virus could not be and is not worse. It's a more general point that suggesting an angle of research not be attempted because the result might be unflattering regarding vaccines is ill advised.

If an individual is not at risk for serious complications from COVID (e.g. healthy, young, and fit) then it's not irrational to consider the potential side effects of a vaccine v.s. just the risk of the disease itself. Even the comparison to the same types of side effects of an actual infection is not a fair one as that assumes the individual is guaranteed to get sick. Not everyone lives in a city and travels in a sardine can on rails to go to work every morning so the number of human contacts and potential infection points is not uniform.

> Scaremongering while putting words into someone else's mouth is why there is mistrust.

Claiming that quoting someone's own words is "putting words in someone else's mouth" is some fantastic double-think.

Re: CoV–2 Spike Impairs DNA Damage Repair – Inhibits V(D)J Recombination in Vitro

#86

Post by pas »

nnx wrote: post #49
LurkingPenguin wrote: post #14

The key finding: > Our findings provide evidence of the spike protein hijacking the DNA damage repair machinery and adaptive immune machinery in vitro. We propose a potential mechanism by which spike proteins may impair adaptive immunity by inhibiting DNA damage repair. Although no evidence has been published that SARS–CoV–2 can infect thymocytes or bone marrow lymphoid cells, our in vitro V(D)J reporter assay shows…

Aren't inactivated virus vaccines (like Sinovac's) also expressing the full-length spike protein?

An inactivated virus does not express anything, it's basically protein debris, and yes, if it's done right it has the full-length spike. But it does not enter cells, it never gets anywhere near DNA.

Re: CoV–2 Spike Impairs DNA Damage Repair – Inhibits V(D)J Recombination in Vitro

#87

Post by pas »

hlieberman wrote: post #34

This is published by a predatory publisher ( https://en.wikipedia.org/wiki/MDPI#Controversial_articles ). According to homozygoat ( https://www.twitch.tv/homozygoat ), it doesn't use real virus (rather, it uses other cells transfected to express some covid proteins), and uses the wrong cell type for the conclusions they try to draw.

Can you link to the actual video where homozygoat discusses this? (And a timestamp too please. Thanks!)

Re: CoV–2 Spike Impairs DNA Damage Repair – Inhibits V(D)J Recombination in Vitro

#88

Post by coldacid »

AstralStorm wrote: post #81
lend000 wrote: post #68

This is one of the more accessible and straightforward microbiology papers I've read. There's no fluff; it tells you how to reproduce and where to buy the materials, and it seems to balance terms in the jargon-heavy field with short explanations, requiring minimal googling to get an overview if you are topically familiar cell biology and chemistry (not to mention VDJ recombination, a fascinating bio-algorithm which s…

The implication being that unmodified spike protein and protein producing vaccines could be less effective than some modified ones and also cause a temporary dip in immunity due to locking out some naive B cells, is that correct?

From my reading, possibly more than just a temporary dip for those who have a severe case of COVID-19.

Re: CoV–2 Spike Impairs DNA Damage Repair – Inhibits V(D)J Recombination in Vitro

#89

Post by dvdhnt »

j16sdiz wrote: post #48
dvdhnt wrote: post #35

Earlier quoted context omitted.

> Thus, even if the vaccine harms you in some way, COVID itself will do worse. And that’s the TL;DR folks. Seriously, this distillation makes the choice very easy.

The choice ain't that simple... There are multiple vaccines.. They varies in effectiveness and safeness. Depends on where you are, the risk of getting inflected varies too. It is good to stay informed, not blindly take anything you can get.

Ehh I didn’t say that did I? The choice I refer to is, get vaccinated or do not.

This sub outsmarts itself so often and it shows. Medical professionals, scientists, researchers, etc are more qualified than I. There’s nothing blind about it.

Re: CoV–2 Spike Impairs DNA Damage Repair – Inhibits V(D)J Recombination in Vitro

#90

Post by bjtitus »

koolba wrote: post #85
revolvingocelot wrote: post #47

Earlier quoted context omitted.

>Explicitly saying nobody should look into a potential negative side effect of vaccines because they might actually find something is down right scary. You didn't read the article. Or what I wrote about it. The vaccine can't do any worse than the virus, and this study, that does look into a potential negative side effect , found that the virus does indeed do worse! Scaremongering while putting words into someone else…

> You didn't read the article. Or what I wrote about it. I literally quoted your words. Are you suggesting I randomly selected text and copy/pasted it without reading it? > The vaccine can't do any worse than the virus, and this study, that does look into a potential negative side effect, found that the virus does indeed do worse! Nothing in what I said implied that the virus could not be and is not worse. It's a mor…

The top 5 states for COVID infection per-capita (https://www.washingtonpost.com/graphics/2020/national/corona...):

North Dakota

Tennessee

Alaska

Wyoming

South Dakota

I don’t believe any of these states even have a single “sardine can on rails” used by daily commuters.

The idea that you are less likely to be infected in rural Wyoming because you commute by car is pure fiction based on the data. Very few people in the United States are able to avoid grocery stores, pharmacies, or restaurants for more than a week or two.

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