11–20 of 111 posts
How much are the flow cells?
Ah. ~$500 - $1000 each. That seems like an expensive consumable, but I have no basis for comparison. Is this a lot? https://store.nanoporetech.com/flowcells.html
https://store.nanoporetech.com/catalog/product/view/id/122/s...
Wow. Can I do whole-genome sequencing with this? Is this a similar product/result as buying whole genome sequencing from an established company for the $500 to $800 they charge these days?
We use ONT's minION for strange niche stuff, where either the DNA prep method matters or the latency (the time from loading to the first data out of the machine) matters. Read quality is typically not as good as illumina, so for applications where we need quality we stick with the NextSeq. There's no reason to think nanopore technology won't get better though, but for now, if you've got the money illumina is still the way to go most of the time.
https://www.nasa.gov/mission_pages/station/research/news/dna...
Wow. Can I do whole-genome sequencing with this? Is this a similar product/result as buying whole genome sequencing from an established company for the $500 to $800 they charge these days?
> You're buying a service for the 500-800$. Here you're buying a flowcell for 1000$ (the "sequencer" is more or less free). The technology is pretty different and a lot more bleeding edge. If what you want is just a genome aligned to a known reference and you can wait a few days then this is not what you want. We use ONT's minION for strange niche stuff, where either the DNA prep method matters or the latency (the time from loading to the first data out of the machine) matters. Read quality is typically not as good as illumina, so for applications where we need quality we stick with the NextSeq. There's no reason to think nanopore technology won't get better though, but for now, if you've got the money illumina is still the way to go most of the time. >
I remember looking into this device before, but as I recall it was intended for small bacterial genomes. A quick Google turns up that a paper using the device to sequence a human genome has only just been published [0] hence the update to the website. I'm not at all familiar with the technology, but doesn't the fact that experts have only just now been able to sequence human DNA mean it would be pretty unlikely the d…
I remember looking into this device before, but as I recall it was intended for small bacterial genomes. A quick Google turns up that a paper using the device to sequence a human genome has only just been published [0] hence the update to the website. I'm not at all familiar with the technology, but doesn't the fact that experts have only just now been able to sequence human DNA mean it would be pretty unlikely the d…
Earlier quoted context omitted.
Ah. ~$500 - $1000 each. That seems like an expensive consumable, but I have no basis for comparison. Is this a lot? https://store.nanoporetech.com/flowcells.html
The washing procedure is a simple 2-step process and the kit contains buffers to remove the previous library and equilibrate for reuse or storage. There are sufficient reagents supplied for 12 washes. https://store.nanoporetech.com/catalog/product/view/id/122/s...
And in my experience, the washing buffer doesn't do a great job. I read somewhere there is ~10% carryover.
I just ran a couple of flowcells last week. I've ran 8 total so far now. My impression is that it can be very inconsistent depending on the quality of the flowcell that they send you and your DNA prep. Great for smallish genomes (bacteria), not enough throughput/quality for large eukaryotic genomes (1gb+) unless you got money to burn.
Are you using it for fun? professionally? academically?