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Immunity Project – HIV Vaccine Development Program [pdf]

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Re: Immunity Project – HIV Vaccine Development Program [pdf]

#41

Post by limpon »

reidrubsamen wrote: post #11
rgejman wrote: post #3

Some thoughts on this white paper, with the caveat that I'm not sure how much of what is discussed they have done and how much of it is what they are planning to do. The white paper is light on details and I re-iterate my appeal for them to release their data (at least in summary form). My apologies for some of the technical language--I want to write this first and then I can clarify the hard stuff (or you can google…

Hi there, Reid from Immunity Project. Thanks for the great question! Would love to talk with you off line about your research, by the way. 1. We have successfully gotten an immune response with memory (by elispot) after a single dose of the PLGA microspheres containing one or two peptides when combined with TLR-4 (MPLA) and TLR-9 (CpG) agonists. We used H2d restricted epitopes to do the study in C57BL/6 mice – this s…

Hi Reid, as I said before, I wish you all the best but I simply don't understand how your experiment 2 is going to work. Did I understand correctly that you will immunize mice and then isolate CD4 T cells from the spleen. Then infect those cells and see if cells from immunized mice die slower/less/not at all and don't express p24 in high levels. I'm afraid this experiment will not work this way. CD4 T cells by itself can not be immunized against HIV. The immunizing effect comes from memory B cells that produce antibodies. These antibodies bind to HIV infected CD4 T cells and as such label them as evil to be killed by other cells. CD4 T cells alone will not show any effect in regards to susceptibility to HIV no matter how well your immunization works. As rgejman pointed out, it would be way better to infect the mice with HIV and do a CD4 T cell count there every week. This would be so much easier and it might actually show you something relevant.

Re: Immunity Project – HIV Vaccine Development Program [pdf]

#42

Post by limpon »

ciokan wrote: post #22

I would be very curious to know if the team received any kind of pressure to stop what they're doing or if they will communicate if such thing would happen. Don't want to sound cynical, I just watched a lot on the subject recently and it seems there's a huge industry behind having a lot more benefits (material ones) from the actual sick people and their life-long expensive treatments compared to a vaccine. Even if it…

I've heard lots of people speculating that the big pharma companies would be better of if there would always be HIV and they can sell their ART pills for live. But on the other hand if one of them is the first one to create a HIV immunization, this alone will make them so extremely rich. An effective HIV immunization will become standard immunization for all people in the western world and the WHO and UN will take all in their power to immunize as many people worldwide as possible. Just imagine the amount of doses that will have to be produced. And you would be the only one company having this product. This has way more financial gain that being one of the dozens of companies that produce ART therapies. On top of that a few people working in high positions of big pharma companies are still "nice" people who would actually be proud of curing a disease. Not everyone just counts their dollars every day and hopes that more people get sick.

Re: Immunity Project – HIV Vaccine Development Program [pdf]

#43

Post by rgejman »

limpon wrote: post #41
reidrubsamen wrote: post #11

Earlier quoted context omitted.

Hi there, Reid from Immunity Project. Thanks for the great question! Would love to talk with you off line about your research, by the way. 1. We have successfully gotten an immune response with memory (by elispot) after a single dose of the PLGA microspheres containing one or two peptides when combined with TLR-4 (MPLA) and TLR-9 (CpG) agonists. We used H2d restricted epitopes to do the study in C57BL/6 mice – this s…

Hi Reid, as I said before, I wish you all the best but I simply don't understand how your experiment 2 is going to work. Did I understand correctly that you will immunize mice and then isolate CD4 T cells from the spleen. Then infect those cells and see if cells from immunized mice die slower/less/not at all and don't express p24 in high levels. I'm afraid this experiment will not work this way. CD4 T cells by itself…

I think that they will incubate CD8 and CD4 T cells from the same mouse together. The CD8 (cytotoxic) T cells, if they recognize the antigen, should kill the infected CD4 cells.

A caveat is activation/co-stimulation. The CD8 T cells should already be activated due to the vaccination and no longer need co-stimulation from DCs... but this depends on the vaccine having delivered enough peptide to stimulate a true immune response in a human/humanized immune system. That's the (first) big unknown here.

Re: Immunity Project – HIV Vaccine Development Program [pdf]

#44

Post by [deleted] »

rgejman wrote: post #43
limpon wrote: post #41

Earlier quoted context omitted.

Hi Reid, as I said before, I wish you all the best but I simply don't understand how your experiment 2 is going to work. Did I understand correctly that you will immunize mice and then isolate CD4 T cells from the spleen. Then infect those cells and see if cells from immunized mice die slower/less/not at all and don't express p24 in high levels. I'm afraid this experiment will not work this way. CD4 T cells by itself…

I think that they will incubate CD8 and CD4 T cells from the same mouse together. The CD8 (cytotoxic) T cells, if they recognize the antigen, should kill the infected CD4 cells. A caveat is activation/co-stimulation. The CD8 T cells should already be activated due to the vaccination and no longer need co-stimulation from DCs... but this depends on the vaccine having delivered enough peptide to stimulate a true immune…

[deleted]

Re: Immunity Project – HIV Vaccine Development Program [pdf]

#45

Post by ciokan »

limpon wrote: post #42
ciokan wrote: post #22

I would be very curious to know if the team received any kind of pressure to stop what they're doing or if they will communicate if such thing would happen. Don't want to sound cynical, I just watched a lot on the subject recently and it seems there's a huge industry behind having a lot more benefits (material ones) from the actual sick people and their life-long expensive treatments compared to a vaccine. Even if it…

I've heard lots of people speculating that the big pharma companies would be better of if there would always be HIV and they can sell their ART pills for live. But on the other hand if one of them is the first one to create a HIV immunization, this alone will make them so extremely rich. An effective HIV immunization will become standard immunization for all people in the western world and the WHO and UN will take al…

yes but op website says they will release it for free, thats why i'm asking in the first place.

Re: Immunity Project – HIV Vaccine Development Program [pdf]

#46

Post by jarrodk »

icinnamon wrote: post #20
shiven wrote: post #15

Earlier quoted context omitted.

Wait! But what HIV protein(s) is the peptide(s) derived from? Let's not overlook the decades of prior data! The field is littered with names like VRC01, Camelid and so many other players. I know, I know, antibodies all versus antigen in this case. But keep reading ... HIV is a master at developing resistant forms of itself. What if it changes the epitope and evades the vaccine induced antibody(ies), again!?! (Edit: D…

Hey shiven! Thanks for your question. This is Ian Cinnamon from the Immunity Project team. The targets we are trying to immunize with are believed to be beneficial by a statistical analysis similar to what is described here: Mothe B, Anuska L, Ibarrondo J, Daniels M, Miranda C, Zamarreno J, et al. Definition of the viral targets of protective HIV-1-specific T cell responses. Journal of Translational Medicine 2011;9(2…

How can you possibly say that resistance may be limited without further clinical tests, but have a project literally stating you can cure HIV - and distribute it for free?
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