A human embryonic limb cell atlas resolved in space and time
1–10 of 13 posts
Re: A human embryonic limb cell atlas resolved in space and time
#2previously: https://news.ycombinator.com/item?id=38559128
Re: A human embryonic limb cell atlas resolved in space and time
#3previously: https://news.ycombinator.com/item?id=38559128
I'll save everyone a click: there's zero comments in the previous submission, which was by the commenter.
Re: A human embryonic limb cell atlas resolved in space and time
#4previously: https://news.ycombinator.com/item?id=38559128
[deleted]
Re: A human embryonic limb cell atlas resolved in space and time
#5OK, I guess I'll be the first to ask. What does this mean?
Re: A human embryonic limb cell atlas resolved in space and time
#6[flagged]
Re: A human embryonic limb cell atlas resolved in space and time
#7OK, I guess I'll be the first to ask. What does this mean?
A cell atlas is a collection of annotated cell types, clustered by similar gene expression. The proximity of the cell types as shown by the projection infer lineage, meaning one cell type begat another. By taking samples at various time points, you can unearth more and more (rare) cell types transitioning from cell type to another, giving you lineage specification from stem cells all the way down to the mature cell types of your tissue of choice. Here they went out on a limb and went with limb.
Re: A human embryonic limb cell atlas resolved in space and time
#8OK, I guess I'll be the first to ask. What does this mean?
A cell atlas is a collection of annotated cell types, clustered by similar gene expression. The proximity of the cell types as shown by the projection infer lineage, meaning one cell type begat another. By taking samples at various time points, you can unearth more and more (rare) cell types transitioning from cell type to another, giving you lineage specification from stem cells all the way down to the mature cell t…
Wow, that's pretty cool. Do we have a complete lineage for any other mature tissue/cell types?
Re: A human embryonic limb cell atlas resolved in space and time
#9Earlier quoted context omitted.
A cell atlas is a collection of annotated cell types, clustered by similar gene expression. The proximity of the cell types as shown by the projection infer lineage, meaning one cell type begat another. By taking samples at various time points, you can unearth more and more (rare) cell types transitioning from cell type to another, giving you lineage specification from stem cells all the way down to the mature cell t…
Wow, that's pretty cool. Do we have a complete lineage for any other mature tissue/cell types?
Loads: blood, brain, (mouse) gastruloids, and many others.
It's not as exciting as a breakthrough as it sounds though. One lab was rich enough to buy the existing tech to sequence it all, and the analytical pipelines are mature enough to do the clustering and lineage tracing for you. Overcoming the batch effects and integrating the different data into each other is still a legitimate struggle, so props to them on that.
Essentially, this a "FIRST!" kind of post, without much science behind it, but it does allow others to tease out new development pathways from the same data, and it acts as a control dataset for others who study mutants which usually have a gene or two knocked out.
The knockouts are where the real science is, but I'm possibly gatekeeping in those last two paragraphs there.
Re: A human embryonic limb cell atlas resolved in space and time
#10Earlier quoted context omitted.
Wow, that's pretty cool. Do we have a complete lineage for any other mature tissue/cell types?
Loads: blood, brain, (mouse) gastruloids, and many others. It's not as exciting as a breakthrough as it sounds though. One lab was rich enough to buy the existing tech to sequence it all, and the analytical pipelines are mature enough to do the clustering and lineage tracing for you. Overcoming the batch effects and integrating the different data into each other is still a legitimate struggle, so props to them on tha…
I'd say you're pushing from flippant to disrespectful on the who and how of this being done. Starting with you've got Addenbrooks and Guangzhou hospitals collecting the foetal material that will have been lovingly donated from some of the most distressed members of the British and Chinese population. The pipeline although commodity came from a new startup Enhanc3D Genomics Ltd (inc. 2020). Over 15 labs worked collaboratively to build the atlas of results, because the expense in human time alone far exceeds what could be done in a single lab.