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Why does the SARS-Cov2 genome end in aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa? (2020)

bioinformatics.stackexchange.com

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Re: Why does the SARS-Cov2 genome end in aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa? (2020)

#121

Post by subroutine »

kevviiinn wrote: post #103
kolinko wrote: post #96

Earlier quoted context omitted.

In this case it probably works most reliably with around this number of As, and less/more would decrease reliability, butnitmwould still be functional. Most of genetics is like that.

With more As you risk running into the upper limit on sequence length for the virus shell and with less you run the risk of quicker degradation and not enough expression

The first point being only a concern if you are a virus. If instead you are engineering say, an rna vaccine, you could package your transcript into lipofectamine instead of a coronavirus envelope ;) but point taken, there's always an upper limit.

Re: Why does the SARS-Cov2 genome end in aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa? (2020)

#122

Post by kevviiinn »

subroutine wrote: post #121
kevviiinn wrote: post #103

Earlier quoted context omitted.

With more As you risk running into the upper limit on sequence length for the virus shell and with less you run the risk of quicker degradation and not enough expression

The first point being only a concern if you are a virus. If instead you are engineering say, an rna vaccine, you could package your transcript into lipofectamine instead of a coronavirus envelope ;) but point taken, there's always an upper limit.

Also a concern when engineering viruses for research or other treatments such as the AAVs

Re: Why does the SARS-Cov2 genome end in aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa? (2020)

#123

Post by sublinear »

eropple wrote: post #112
sublinear wrote: post #101

Earlier quoted context omitted.

I think you'll find similar bullshittery in the details of every topic that isn't math. Think of it like finding "TODO" comments in old code.

I mean, historically mathematics have had areas full of "HACK:" comments, too--ones retroactively applied to the whole of Newtonian physics, even, but it's still useful enough that we keep it around!

All I meant is that the approximations in math are in the assumptions which is far easier to deal with in the grander scheme of things.

Re: Why does the SARS-Cov2 genome end in aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa? (2020)

#124

Post by eropple »

sublinear wrote: post #123
eropple wrote: post #112

Earlier quoted context omitted.

I mean, historically mathematics have had areas full of "HACK:" comments, too--ones retroactively applied to the whole of Newtonian physics, even, but it's still useful enough that we keep it around!

All I meant is that the approximations in math are in the assumptions which is far easier to deal with in the grander scheme of things.

Assumptions? Don't worry; we've got those too! if I had a nickel for all the times I've seen somebody pick 1 for "0, 1, is N" input cases? ;)

Re: Why does the SARS-Cov2 genome end in aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa? (2020)

#125

Post by subroutine »

kevviiinn wrote: post #122
subroutine wrote: post #121

Earlier quoted context omitted.

The first point being only a concern if you are a virus. If instead you are engineering say, an rna vaccine, you could package your transcript into lipofectamine instead of a coronavirus envelope ;) but point taken, there's always an upper limit.

Also a concern when engineering viruses for research or other treatments such as the AAVs

True. I should have said "if you are using a virus"
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