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‘Encrypted’ peptides could be wellspring of natural antibiotics

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Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#21

Post by noduerme »

cing wrote: post #13
ackbar03 wrote: post #2

Can anyone shed light on how complicated, slow, and costly it is to synthesis these proteins in vitro?

Our startup routinely orders the synthesis of hundreds of peptides for technology validation and drug discovery research (not suitable for human consumption). Costs for a small quantity through a contract research organizion can be $200-$2000 USD per peptide depending on desired purity, length, and chemical complexity. For some applications peptide arrays are suitable, and can drive the costs down to $10 USD per pept…

How do you verify they got the structure right? If it's like everything else you order online these days...

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#22

Post by radicaldreamer »

nikkwong wrote: post #18
yosito wrote: post #15

Earlier quoted context omitted.

I've got similar health problems, and I'd love to know more about what worked for you so I can research it.

Thanks; I'm sorry to hear about your health problems I know how debilitating they can be. Hope your case is not as bad as mine. I'm currently self administering FMTs which I get from someone in my local community. I have tested the stool of dozens of people (which is so expensive) and have settled on this donor who has a decent stool profile. An alternative to that may be trying to get stool from humanmicrobes.org or…

Does BPC-157 have to be injected? (I thought peptides were fragile and need to be refrigerated typically)

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#23

Post by asdff »

robbiep wrote: post #7
dnautics wrote: post #4

Earlier quoted context omitted.

By hand a skilled practitioner of t-boc chemistry can couple one amino acid per hour, though t-boc (boc) is disfavored since workup involves using 100% HF. Fmoc is the more popular technique and it's more like two hours. Robots can do this too, there's no real time benefit (but a robot can do more than 10 peptide couplings a day and doesn't need a lunch break -- though I guess neither does a grad student). Chief prob…

I would have imagined labs these days just synthesise the mRNA and then let a ribosome do it’s work?

even better is to put it in yeast or e coli and let them be your factory

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#24

Post by asdff »

noduerme wrote: post #21
cing wrote: post #13

Earlier quoted context omitted.

Our startup routinely orders the synthesis of hundreds of peptides for technology validation and drug discovery research (not suitable for human consumption). Costs for a small quantity through a contract research organizion can be $200-$2000 USD per peptide depending on desired purity, length, and chemical complexity. For some applications peptide arrays are suitable, and can drive the costs down to $10 USD per pept…

How do you verify they got the structure right? If it's like everything else you order online these days...

you can put it on a mass spectrometer

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#25

Post by nikkwong »

radicaldreamer wrote: post #22
nikkwong wrote: post #18

Earlier quoted context omitted.

Thanks; I'm sorry to hear about your health problems I know how debilitating they can be. Hope your case is not as bad as mine. I'm currently self administering FMTs which I get from someone in my local community. I have tested the stool of dozens of people (which is so expensive) and have settled on this donor who has a decent stool profile. An alternative to that may be trying to get stool from humanmicrobes.org or…

Does BPC-157 have to be injected? (I thought peptides were fragile and need to be refrigerated typically)

I don't know the answer to that; I inject mine, I have read that other people squirt them into their mouth, which I have not tried yet. The results are just so good from injecting it that I can't imagine ingesting it orally being better; but I should give it a shot.

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#26

Post by yosito »

hammock wrote: post #5

It is a little known fact that peptides are becoming really big in the athletes/recovery/PED space. There is a lot they can do and there are so many. However we are also learning that they can cause a lot of unintended disease, cancer, etc as well.

> we are also learning that they can cause a lot of unintended disease, cancer, etc

From what I've read, as a layman, many peptides can increase cell growth which is sometimes associated with tumor growth, but they may also have a stabilizing effect on cell growth which wouldn't cause cancer at all, and could even be beneficial. I don't think there has been any evidenevidence linking peptides with cancer though. Would be eager to know if anyone has seen such evidence.

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#27

Post by gabll »

hourislate wrote: post #14

Another interesting Peptide. There is evidence of BPC 157 can help heal IBS, etc. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333585/ It has other healing properties related to ligament injuries. https://pubmed.ncbi.nlm.nih.gov/21030672/ Another study on its benefits regarding blood vessels. https://pubmed.ncbi.nlm.nih.gov/23782145/ An article from Ben Greenfield https://bengreenfieldfitness.com/article/supplements…

Did you find also studies on long term effects? I couldn’t find any

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#28

Post by nikkwong »

hanniabu wrote: post #19
nikkwong wrote: post #12

Earlier quoted context omitted.

It’s scary, but I’m taking some of these myself. I had food poisoning and was diagnosed with IBS-C a few years ago and my life has been pretty deranged since then. Couldn’t sleep more than a few hours a night, couldn’t think, went from a BMI of 22 to 18. No help from doctors, or years of different drug therapies; I’ve tried everything that medicine has to offer. Also could not get my blood pressure down to save my li…

How long did it take you to see results after taking them?

Pretty much right away for both. I think it's because with my condition; eating seems to be incredibly inflammatory. So, whenever I eat anything, I my blood pressure spikes tremendously, heart rate jumps ~15 points, I get brain fog & all kinds of other symptoms, which last several hours only to subside when I start to get really hungry again. Of course.. at that point I'm hungry, and it becomes increasingly hard to not eat.. so once I cave and eat the cycle starts over again. I currently eat twice a day and have tried cutting that down to once a day but at that point my meals are so large that the falllout is just terrible. The goal is always to eat as little as possible while maintaining adequate nutrition, which is hard, because I also don't seem to assimilate nutrients from food very well either.

Anyways, if I am taking a lot of fecal matter and using the peptides; the fallout I get from eating is not as bad. There are still other issues, but it seems to work at at least within a few days with the fecal matter and instantly with the peptides. Very sadly, I've noticed the benefits go away pretty quickly once I stop using each treatment as well. It really is a nightmare, but I am sure others have it worse.

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#29

Post by thriftwy »

hammock wrote: post #5

It is a little known fact that peptides are becoming really big in the athletes/recovery/PED space. There is a lot they can do and there are so many. However we are also learning that they can cause a lot of unintended disease, cancer, etc as well.

Peptides are actually "short bits of protein" - proteins is what makes cell work. So no wonder here, that they are really big and can do a lot.

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#30

Post by temp0826 »

noduerme wrote: post #20

Uh, is there any logical reason to think that the genome of a human - or any other higher level organism - has coded for unique antibiotic combinations that, e.g. various fungi which have spent the last billion years in daily fist fights with bacteria? I'm all for trawling the DNA of any creature for magical recipes, but our DNA has to code for a lot , and immune response is only a small part of it. Seems like it wou…

I wish some money would be thrown at researching Kambo (an amazonian tree frog with a poisonous secretion used by a few indigenous tribes touted for its peptide cocktail, "the vaccine of the forest"), considering its growing use. Seems like a better place to start than some of these stabs in the dark.
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