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‘Encrypted’ peptides could be wellspring of natural antibiotics

blog.seas.upenn.edu

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Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#11

Post by techbio »

dnautics wrote: post #4
ackbar03 wrote: post #2

Can anyone shed light on how complicated, slow, and costly it is to synthesis these proteins in vitro?

By hand a skilled practitioner of t-boc chemistry can couple one amino acid per hour, though t-boc (boc) is disfavored since workup involves using 100% HF. Fmoc is the more popular technique and it's more like two hours. Robots can do this too, there's no real time benefit (but a robot can do more than 10 peptide couplings a day and doesn't need a lunch break -- though I guess neither does a grad student). Chief prob…

I had to Google ubiquitin to verify that this wasn't a dryly elaborate reddit-surprise thread. It is indeed a well-named compound, ubiquitous among eukaryotes, and even if "boc" and "fmoc" are basically just fizz and buzz to me, it sure sounds like you know what you're doing.

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#12

Post by nikkwong »

radicaldreamer wrote: post #6
hammock wrote: post #5

It is a little known fact that peptides are becoming really big in the athletes/recovery/PED space. There is a lot they can do and there are so many. However we are also learning that they can cause a lot of unintended disease, cancer, etc as well.

Yikes, I know there are a lot of "healing" peptides sold online for various conditions. Very spare documentation though

It’s scary, but I’m taking some of these myself. I had food poisoning and was diagnosed with IBS-C a few years ago and my life has been pretty deranged since then. Couldn’t sleep more than a few hours a night, couldn’t think, went from a BMI of 22 to 18. No help from doctors, or years of different drug therapies; I’ve tried everything that medicine has to offer. Also could not get my blood pressure down to save my life even on multiple meds. Fecal matter transplants helped quite a bit but still couldn’t get BP down, get good sleep, etc. Only when I started using some peptide suggested on Reddit was I able to normalize my BP and see improvements in other areas. It’s scary and I don’t recommend it (my doc certainly doesn’t, but he doesn’t endorse any of the therapies that have improved my wellbeing); I don’t want to be a guinea pig for a peptide that has a limited amount of human data to back it up; but the alternative of not using it is just so unlivable and having it solve my BP problem alone is a gift in itself. I hope I’m not shooting my future self in the foot by using these but I don’t see any alternative option currently. It sucks to be sick when medicine still hasn’t figured things out.

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#13

Post by cing »

ackbar03 wrote: post #2

Can anyone shed light on how complicated, slow, and costly it is to synthesis these proteins in vitro?

Our startup routinely orders the synthesis of hundreds of peptides for technology validation and drug discovery research (not suitable for human consumption). Costs for a small quantity through a contract research organizion can be $200-$2000 USD per peptide depending on desired purity, length, and chemical complexity. For some applications peptide arrays are suitable, and can drive the costs down to $10 USD per peptide or lower. In both cases, the turnaround time is 4-6 weeks, even though a peptide chemist could do the job in about half the time for a rush order.

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#14

Post by hourislate »

Another interesting Peptide.

There is evidence of BPC 157 can help heal IBS, etc.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333585/

It has other healing properties related to ligament injuries.

https://pubmed.ncbi.nlm.nih.gov/21030672/

Another study on its benefits regarding blood vessels.

https://pubmed.ncbi.nlm.nih.gov/23782145/

An article from Ben Greenfield

https://bengreenfieldfitness.com/article/supplements-article...

A source for anyone interested.

https://infiniwell.com/products/bpc-157

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#15

Post by yosito »

nikkwong wrote: post #12
radicaldreamer wrote: post #6

Earlier quoted context omitted.

Yikes, I know there are a lot of "healing" peptides sold online for various conditions. Very spare documentation though

It’s scary, but I’m taking some of these myself. I had food poisoning and was diagnosed with IBS-C a few years ago and my life has been pretty deranged since then. Couldn’t sleep more than a few hours a night, couldn’t think, went from a BMI of 22 to 18. No help from doctors, or years of different drug therapies; I’ve tried everything that medicine has to offer. Also could not get my blood pressure down to save my li…

I've got similar health problems, and I'd love to know more about what worked for you so I can research it.

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#16

Post by [deleted] »

dnautics wrote: post #4
ackbar03 wrote: post #2

Can anyone shed light on how complicated, slow, and costly it is to synthesis these proteins in vitro?

By hand a skilled practitioner of t-boc chemistry can couple one amino acid per hour, though t-boc (boc) is disfavored since workup involves using 100% HF. Fmoc is the more popular technique and it's more like two hours. Robots can do this too, there's no real time benefit (but a robot can do more than 10 peptide couplings a day and doesn't need a lunch break -- though I guess neither does a grad student). Chief prob…

[deleted]

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#17

Post by The_rationalist »

yosito wrote: post #15
nikkwong wrote: post #12

Earlier quoted context omitted.

It’s scary, but I’m taking some of these myself. I had food poisoning and was diagnosed with IBS-C a few years ago and my life has been pretty deranged since then. Couldn’t sleep more than a few hours a night, couldn’t think, went from a BMI of 22 to 18. No help from doctors, or years of different drug therapies; I’ve tried everything that medicine has to offer. Also could not get my blood pressure down to save my li…

I've got similar health problems, and I'd love to know more about what worked for you so I can research it.

He is probably talking about BPC 157

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#18

Post by nikkwong »

yosito wrote: post #15
nikkwong wrote: post #12

Earlier quoted context omitted.

It’s scary, but I’m taking some of these myself. I had food poisoning and was diagnosed with IBS-C a few years ago and my life has been pretty deranged since then. Couldn’t sleep more than a few hours a night, couldn’t think, went from a BMI of 22 to 18. No help from doctors, or years of different drug therapies; I’ve tried everything that medicine has to offer. Also could not get my blood pressure down to save my li…

I've got similar health problems, and I'd love to know more about what worked for you so I can research it.

Thanks; I'm sorry to hear about your health problems I know how debilitating they can be. Hope your case is not as bad as mine. I'm currently self administering FMTs which I get from someone in my local community. I have tested the stool of dozens of people (which is so expensive) and have settled on this donor who has a decent stool profile.

An alternative to that may be trying to get stool from humanmicrobes.org or a more "legit" way which is through the center for digestive diseases in Australia. I don't know if FMT's are helpful for all IBS patients (the research is not there yet) but anecdotally it seems like the most desperate IBS patients end up trying it to varying efficacy. I can't recommend FMT as I'm sure everyone's pathology is different and there are more traditional therapies that may work such as low FODMAP, rifaximin, or meds that increase/decrease intestinal transit time. For me, all of those therapies are embarrassingly ineffective and I think that IBS patients should at least know that FMT therapy is a thing; since it has lead to remission for many community members which gives hope that there may finally be a treatment for the most desperate.

So the FMT helps in my case as well as the BPC-157 which someone else linked in the comments. I was quite surprised when my resting heart rate and blood pressure returned to my pre-disease levels within a day or two of starting the peptides. I have probably tried 3 or 4 dozen drugs at this point (mostly doctor recommended but also some from my own research); none to any effect, so seeing quick results from this new compound was shocking. I now also have more of type 2-3 stools rather than the unescapable type 1 that has plagued me ever since the diseases' inception. So that's good. I've only been using the peptides for a month so don't know what to expect. I am hoping if I can mega dose with some FMT and keep using the peptides maybe I could get into remission. Who knows. Luckily I am able to function as an engineer again for the first time in years (the brain fog is less remarkable now) so I am hoping things will return to normal but only time will tell. I hope this information is helpful but feel free to reach out if you have questions or just would like to chat because I know how lonely and lost in translation these type of health maladies can be.

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#19

Post by hanniabu »

nikkwong wrote: post #12
radicaldreamer wrote: post #6

Earlier quoted context omitted.

Yikes, I know there are a lot of "healing" peptides sold online for various conditions. Very spare documentation though

It’s scary, but I’m taking some of these myself. I had food poisoning and was diagnosed with IBS-C a few years ago and my life has been pretty deranged since then. Couldn’t sleep more than a few hours a night, couldn’t think, went from a BMI of 22 to 18. No help from doctors, or years of different drug therapies; I’ve tried everything that medicine has to offer. Also could not get my blood pressure down to save my li…

How long did it take you to see results after taking them?

Re: ‘Encrypted’ peptides could be wellspring of natural antibiotics

#20

Post by noduerme »

Uh, is there any logical reason to think that the genome of a human - or any other higher level organism - has coded for unique antibiotic combinations that, e.g. various fungi which have spent the last billion years in daily fist fights with bacteria? I'm all for trawling the DNA of any creature for magical recipes, but our DNA has to code for a lot, and immune response is only a small part of it. Seems like it would be a lot more efficient to focus on phages or fungi and study what peptides they activate in response to specific bacteria, than just throwing endless processing cycles at the human genome hoping for, uh, grant money?
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