The impure peptides is the part that scares me. What could be in there ? Could you end up moredead? The nasal pathway is quite direct I gather.
Stay safe ! Cheers
Making Vaccine
221–230 of 378 posts
Re: Making Vaccine
#222Inhaling peptides is not a vaccine unless they are using an adjuvant. You need something to kick off the immune response and recognize the peptides as associated with a pathogen, otherwise you’d be getting immune responses to everything you inhale in the environment (like when you cook).
And if they are using adjuvants, well then as others have posted.. autoimmune issues can be real.
It’s not that it’s not possible... just something you might want to try on a mouse before up your nose. Why oh why experiments on yourself or suggest this to others without lots of warnings?
Re: Making Vaccine
#223Order peptides they say? Won't scale in a pandemic. And in regular times we can get vaccines no problem.
Re: Making Vaccine
#224Earlier quoted context omitted.
Honestly i'd be pretty shocked if this actually worked.
Why? In many cases, the hard part isn't so much making a working vaccine, as proving it works, scaling manufacturing, etc.
Have you made a successful vaccine to a major virus before in your basement?
Re: Making Vaccine
#225Earlier quoted context omitted.
Why? In many cases, the hard part isn't so much making a working vaccine, as proving it works, scaling manufacturing, etc.
Because if all it took to make a vaccine for a respiratory disease was stick some peptides up your nose, we would havev a lot more vaccines (and i dont mean just for covid.). Even if the other parts are harder (not sure if thats true. The reason proving things work is hard is because most initially promising candidates dont actually), it doesn't follow that every single vaccine candidate works.
Kind of like self driving cars, so many edge cases, so many unknowns in deployment. Got to test the shit out of it.
Re: Making Vaccine
#226Earlier quoted context omitted.
In order to use this method to test a vaccine for deployment to people over 65, you need people over 65 in your control group. This means you are choosing people to murder.
Do separate branches then. Use challenge trials to accelerate deployment to under 30s (or whatever) and natural infection for other age brackets. I honestly haven't thought deeply about the ethics, but it's really concerning that it has become such a hard rule that preventing people from injuring themselves in a particular way (we don't prevent lots of other forms of injury) is somehow obviously superior to letting t…
> I honestly haven't thought deeply about the ethics
"but here's my half-baked opinion anyway"
Re: Making Vaccine
#227Similar to the author of the post, I'm surprised that I haven't seen or heard much about bio-hacking your own vaccine. Seems like an interesting trend to follow, though I will be waiting for an FDA-approved vaccine myself. I am a little worried that https://radvac.org/vaccine/ mentions that their vaccine has "the most extreme complication in some recipients of stuffy noses." I would expect a functioning vaccine to el…
> bio hacking your own vaccine. Seems like an interesting trend to follow
There's only one of you, no second chances if you mess up.
Re: Making Vaccine
#228Curious whether short peptides are sufficient to generate a formidable immune response. Looking at the paper cited, it's not trivial to detect T-cell immunity so I'm not sure a general antibody test would be able to pick up an immune response. It's probably better than doing nothing, but it's not clear whether it's even in the same ballpark as a Pfizer vaccine. edit: I'm not sure about t-cell vs b-cell responses in i…
They’re not sufficient without an adjuvant. Peptides by themselves usually aren’t enough to be recognized as pathogenic. If any peptide by itself could kick off an immune response, you’d be getting sick with everything you eat and smell.
Re: Making Vaccine
#229This article could have been written by Gwyneth Paltrow and it would have had the same level of authority. There's a reason vaccines require staged trials. This seems play playing not with fire, but with nitroglycerin or maybe Nitrogen triiodide.
Now you mention it, I do wonder how this exact same article would have been received on HN if it had been written by Gwyneth Paltrow.
Re: Making Vaccine
#230Earlier quoted context omitted.
>Preston Estep, Don Wang, Alex Hoekstra, Ranjan Ahuja, George Church, Brian Delaney, and over 100 others Pretty sure George Church is a "formally trained scientist"!
Dude, Pfizer can make a mRNA vaccine but that doesn't mean if I follow their directions I can. This guy is a total amateur. He bought some proteins, diluted them then shot them in his nose. That's not science. And I also question his methods. Simply shooting a small peptide into your nose is unlikely to elicit any major immune response. Cells in your respiratory tract excrete proteases. I'm guessing those peptides ge…
He used chitosan nanoparticle as delivery mechanism, which has been validated in multiple clinical trials.