A CRISPR first produces squid as clear as glass
1–10 of 71 posts
Re: A CRISPR first produces squid as clear as glass
#2For anyone coming to the comments before reading the article: the squid is already clear, they just removed some spots of pigment.
Re: A CRISPR first produces squid as clear as glass
#3For anyone coming to the comments before reading the article: the squid is already clear, they just removed some spots of pigment.
Thank you! I don't like admitting to this behavior, but I can't trust a headline (any headline).
Re: A CRISPR first produces squid as clear as glass
#4For anyone coming to the comments before reading the article: the squid is already clear, they just removed some spots of pigment.
The reason this is a significant achievement is that the DNA of living cells in the organism were "edited" to remove the pigmentation, thereby effectively changing the phenotype of an adult squid.
Re: A CRISPR first produces squid as clear as glass
#5For anyone coming to the comments before reading the article: the squid is already clear, they just removed some spots of pigment.
And the advantage here is that it's the start of development of a genetic-modification toolkit (similar to that we already have for fruit flies and c elegans and zebrafish) for using them as animal models. Much more complex of a brain than any of the other animal models we usually use.
Re: A CRISPR first produces squid as clear as glass
#6I feel creeped out/put off about this. These are intelligent beings, apparently limited only by their short lifespan.
They communicate by modulating the pigments into patterns.
Now that has been taken away. Imagine toasting some genes in a human embryo to the effect of lock in syndrome. Crispy!
edit: I think this is a case for PETA to look into.
Re: A CRISPR first produces squid as clear as glass
#7For anyone coming to the comments before reading the article: the squid is already clear, they just removed some spots of pigment.
The reason this is a significant achievement is that the DNA of living cells in the organism were "edited" to remove the pigmentation, thereby effectively changing the phenotype of an adult squid.
They edited embryos:
we determined whether a TDO-selective inhibitor (680C91) impeded pigmentation in developing embryos. The development of D. pealeii has been divided into 30 stages and eye pigmentation appears at ∼stage 25, while chromatophore pigmentation starts at stage 26 [21]. We added 680C91 to developing embryos at stage 20
Re: A CRISPR first produces squid as clear as glass
#8IIRC it's a big deal because this is the first crack at cephalopod DNA - I don't recall where I read this. Anyone has a source?
Re: A CRISPR first produces squid as clear as glass
#9Earlier quoted context omitted.
The reason this is a significant achievement is that the DNA of living cells in the organism were "edited" to remove the pigmentation, thereby effectively changing the phenotype of an adult squid.
They edited embryos: we determined whether a TDO-selective inhibitor (680C91) impeded pigmentation in developing embryos. The development of D. pealeii has been divided into 30 stages and eye pigmentation appears at ∼stage 25, while chromatophore pigmentation starts at stage 26 [21]. We added 680C91 to developing embryos at stage 20
While you're right they edited the embryos the sentence you quoted is describing a drug (680C91) that they bath-applied to inhibit the TDO enzyme (as a positive control), rather than the CRISPR strategy they used, in which they injected the Cas9 and sgRNA into the embryo.
Re: A CRISPR first produces squid as clear as glass
#10As a learner, is it possible to self learn more about Gene Editing and these things, and can these things can be learned in a hacker way without going through a formal academic route ?