Viewing profile — theddman

That's a great explanation! To add a cool point, the wobble position is frequently modified by highly specific enzymes to make it matter more. It's like some random protein mutated…

What do you mean synthesize? If you're starting from GHB, then you just titrate up with NaOH to ~neutral?

This article doesn't touch on how they also used mRNA coding for Cas9 instead of the protein itself.