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when i looked i was interested, but was turned off when i saw that the cost far outstripped commercial sequencing services.
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Note that you are literally shedding identifiable DNA from your body at all times and a truly motivated adversary would have no problem obtaining enough sample material to do high quality sequencing.
At scale?
Edit: I used to help Google fund researchers like Joe Derisi and others who develop technology to do this, and some of the people I worked with in my academic career are quite good at identifying serial killers from 30 year old DNA. If you're downvoting because you think I'm making this up, you're wrong. If you're downvoting because you don't think large-scale individual detection using genetic sampling of the environment is possible, you're wrong. If you're downvoting because you think you couldn't do a whole genome sequence of an individual using a sample collected in the wild, you're wrong. If you're downvoting because you think this is a terrible idea (morally, ethically), that's fine but I didn't say anything about my own moral or ethical beliefs about this.
It's simply factually correct to say that large-scale individual sample collection (at order tens of thousands, if not hundreds of thousands of individuals in a country the size of the US) is possible. All the technology is there to do this.
I'm really curious about what I could learn by getting my DNA sequenced, but I'm worried about my rights to not have it recorded and shared without my consent if I got someone else to do it for me - so any advance toward an affordable home test setup is very welcome.
Imagine insurers refusing to give you a service due to your predisposition to certain diseases...
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Yep that’s the one. They update the flow cells over time. The bit they don’t tell you is the stuff you need, like a qubit, to properly run the thing.
A qubit or fluorometer isn't required. You can use a simple DNA ladder to measure the relative quantity and quality of DNA that's good enough for nanopore sequencing. I just did a full genome sequence of a novel fungus using this exact approach.
You also still, in that case, need a gelbox + ladder + loading dye + sybrsafe or whatever, so it’s still not nothing.
I'm really curious about what I could learn by getting my DNA sequenced, but I'm worried about my rights to not have it recorded and shared without my consent if I got someone else to do it for me - so any advance toward an affordable home test setup is very welcome.
Note that you are literally shedding identifiable DNA from your body at all times and a truly motivated adversary would have no problem obtaining enough sample material to do high quality sequencing.
DNA sequencing bugs me quite a bit. On one hand, I would love to learn something new about my body. On the other hand, what if the results tell me that I am predisposed to some horrible untreatable disease? Will I spend the rest of my days observing every little pain or discomfort and thinking "is this IT?"
DNA sequencing bugs me quite a bit. On one hand, I would love to learn something new about my body. On the other hand, what if the results tell me that I am predisposed to some horrible untreatable disease? Will I spend the rest of my days observing every little pain or discomfort and thinking "is this IT?"
How about affinities to possible health issues, which could be avoided if you started now and not in 20 years?
Perhaps a trusted middleman would be a solution: "just don't tell me about anything that is totally beyond my control".
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I recommend reading this review https://genomebiology.biomedcentral.com/articles/10.1186/s13... I guess there are limits to ensemble methods if the underlying accuracy doesn't increase. I don't work on gene sequencing algorithms but from what I understand of ML ensemble techniques, there are certain assumptions regarding the underlying independence of the errors. The errors for nanopore should be uniform but I am not…
I know that the error rate of the oxford nanopore sequencer depends on GC content (guanine/cytosine nucleotides), and that the Pacific Biosciences sequencer uses a polymerase that gets worn down during reading. So there is some non-uniformity in the chemistry.
So, how long before I can take my DNA "ROM" file and boot it in an emulator that would allow it to grow?
it's unlikely we would ever be able to achieve this. Even simulating a single cell at high resolution is a serious challenge.
What was unthinkable 50 years ago, playing chess better than a human, it's now trivial for a $100 device.
And it's not necessarily required that to simulate the growth of a human you'll need to simulate the entirety of chemical reactions in all 50 trillion cells and all that.
I'm really curious about what I could learn by getting my DNA sequenced, but I'm worried about my rights to not have it recorded and shared without my consent if I got someone else to do it for me - so any advance toward an affordable home test setup is very welcome.
Imagine insurers refusing to give you a service due to your predisposition to certain diseases...