Earlier quoted context omitted.
No, because the cashiers handling smaller transactions finish quicker and can process more people. If you're stuck behind a guy which takes 2 minutes to check out, people in other lanes will be in their cars before you even begin checking out.
Here's a concrete example to make harabago's point clearer (since I think it is a little tricky). Imagine 9 people in line. Each person takes 1m to checkout except the first guy takes 10m. With three lines it looks like this (these are their wait times before being serviced): 0 0 0 (first person in each line has no wait time) 10 1 1 11 2 2 If you sum up the total wait times you get: 27m Now do this with a single file…
If they do that, they'll each wait for 3 minutes, giving the same distribution as in the single line.
Ah, you say, but what if other people have entered those lines already? Then you have to count their wait times too, which will be shorter in the three-line case than the one-line case.
Using one line doesn't reduce the mean wait time; it's always the number of people waiting divided by the throughput. What it reduces is the variance in wait times, making the system more fair.