I don't see any reference to the "USB dongle" mentioned in the title. I was thinking this would be some cool thing you could do at home.
Sequencing your DNA with a USB dongle and open source code
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Re: Sequencing your DNA with a USB dongle and open source code
#42Earlier quoted context omitted.
A used laboratory grade NGS system can be had for less than 10K https://www.ebay.com/itm/265148387179 Nanopore is still not quite ready yet for precise and high accuracy sequencing. Give it another five years.
wow i didn’t know they were that “cheap” now. i used to work for a major competitor to the sequencer you linked, the SOLiD. and i feel like nanopore is the VR of dna sequencing. it’s always just another few years off.
What do you mean by it's always a few years off? Nanopore will allow you to do high-quality genomic sequencing _now_, in a home lab if you wanted, for less than $3K. If you amortize the 3K by the number of genomes you can sequence on the same flow cell, the price per base or per genome falls precipitously, depending on the size of the genome of course.
Re: Sequencing your DNA with a USB dongle and open source code
#43I don't see any reference to the "USB dongle" mentioned in the title. I was thinking this would be some cool thing you could do at home.
Re: Sequencing your DNA with a USB dongle and open source code
#44DNA sequencing bugs me quite a bit.
On one hand, I would love to learn something new about my body.
On the other hand, what if the results tell me that I am predisposed to some horrible untreatable disease? Will I spend the rest of my days observing every little pain or discomfort and thinking "is this IT?"
Re: Sequencing your DNA with a USB dongle and open source code
#45So, how long before I can take my DNA "ROM" file and boot it in an emulator that would allow it to grow?
it's unlikely we would ever be able to achieve this. Even simulating a single cell at high resolution is a serious challenge.
Re: Sequencing your DNA with a USB dongle and open source code
#46Folks are free to analyze my genome, https://my.pgp-hms.org/profile/hu80855C
Last time it was analyzed the conclusion was that there was nothing actionable.
Re: Sequencing your DNA with a USB dongle and open source code
#47Nanopore sequencing is a really interesting technology. It utilizes fundamentally the same apparatus as a Coulter Counter [1], which is a general method of counting and sizing arbitrary particles that's frequently used in flow cytometry. Applying it to sequencing by drawing unwound DNA through the pore was a really excellent logical leap, and we're only now starting to see the benefits of even though it was first ideated over 30 years ago.
Re: Sequencing your DNA with a USB dongle and open source code
#48I'm really curious about what I could learn by getting my DNA sequenced, but I'm worried about my rights to not have it recorded and shared without my consent if I got someone else to do it for me - so any advance toward an affordable home test setup is very welcome.
Note that you are literally shedding identifiable DNA from your body at all times and a truly motivated adversary would have no problem obtaining enough sample material to do high quality sequencing.
Re: Sequencing your DNA with a USB dongle and open source code
#49TMI.
Re: Sequencing your DNA with a USB dongle and open source code
#50I'm really curious about what I could learn by getting my DNA sequenced, but I'm worried about my rights to not have it recorded and shared without my consent if I got someone else to do it for me - so any advance toward an affordable home test setup is very welcome.
Note that you are literally shedding identifiable DNA from your body at all times and a truly motivated adversary would have no problem obtaining enough sample material to do high quality sequencing.
At scale?