Does anyone have links to audio samples where these differences are prominent? In addition to comments, this video has a decent comparison using sawtooth tones: https://www.youtube.com/watch?v=VRlp-OH0OEA
- pure major/minor
- pythagorean
- mean tone
- werckmeister/kirnberger, which i've heard mentioned as a good option for acoustic pianos a few times (i suppose i should try it)
For any besides ET, you have to specify the root/base note.
I think my synths have these options as well, so if you know anybody that has one, you can try it yourself. I think for string instruments, a few cents difference is pretty audible, but not so much for, say clarinet which doesn't have the sharp transients, unless you're playing above the treble clef staff. and then when you hit a track with overdrive/distortion, reverb and delay, it becomes very hard to discern differences.