Earlier quoted context omitted.
https://ansabio.com/ advertising 50 kb
How is ANSA bio? I just got hit up by a recruiter for this company at random, and now I see them mentioned on Hackernews
It's a sign! I'm too biased to give you advice.
Leap in DNA synthesis slashes time to build new genetic sequences
31–38 of 38 posts
Re: Leap in DNA synthesis slashes time to build new genetic sequences
#32Re: Leap in DNA synthesis slashes time to build new genetic sequences
#33This is not a practical challenge - I order DNA from Twist at these ‘large’ scales trivially without needing to do oligo hybridization magic. The DNA arrives in a month - but considering how many oligos sidewinder calls for, not clear how they could be faster.
out of curiosity, what do you do with them? what's your use case for large DNA?
Re: Leap in DNA synthesis slashes time to build new genetic sequences
#34Re: Leap in DNA synthesis slashes time to build new genetic sequences
#35Re: Leap in DNA synthesis slashes time to build new genetic sequences
#36This is not a practical challenge - I order DNA from Twist at these ‘large’ scales trivially without needing to do oligo hybridization magic. The DNA arrives in a month - but considering how many oligos sidewinder calls for, not clear how they could be faster.
out of curiosity, what do you do with them? what's your use case for large DNA?
For me it makes it easier to study long, alternatively spliced genes.
But there are many use cases - you can see them described on vendors’ pages and even in this new post.
Re: Leap in DNA synthesis slashes time to build new genetic sequences
#37This is not a practical challenge - I order DNA from Twist at these ‘large’ scales trivially without needing to do oligo hybridization magic. The DNA arrives in a month - but considering how many oligos sidewinder calls for, not clear how they could be faster.
12kb, you can get three gblocks in a week and change and have them assembled in two days.
Not sure if the oligo approach would circumvent this concern, but I had a GC-content issue with gene blocks that precluded me from using them. Twist was able to do the synthesis, however (not sure what platform they use for their synthesis and why they were able to offer this).
Re: Leap in DNA synthesis slashes time to build new genetic sequences
#38I can't be the only one reading this who doesn't have alarm bells going off in their heads.
I too don't have alarm bells going off.