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Nitrile and latex gloves may cause overestimation of microplastics

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31–40 of 282 posts

Re: Nitrile and latex gloves may cause overestimation of microplastics

#31

So the problem is these particles are literally flying off the gloves of the scientists wearing them to the point it's interfering with the experiment and so... it's less of a problem?

No, the gloves leave stearates (not plastic, but similar looking particles) residue on contact. So there are not literally micro plastics flying off the gloves. Read the article.

Re: Nitrile and latex gloves may cause overestimation of microplastics

#33
post #29

So the problem is these particles are literally flying off the gloves of the scientists wearing them to the point it's interfering with the experiment and so... it's less of a problem?

If you read the article you'd find that what they are finding are not microplastics - they're stearates[1] These are soap-like chemicals used as mould release agents on gloves, but what also means are chemically similar to plastics when analyzed by some techniques and under a microscope will spontaneously form micelle-structures which look very similar to microplastics (you can't exactly get in there and poke them).…

“if you read the article”

Now why would anyone do that when the headline already supports their uninformed opinion?

Re: Nitrile and latex gloves may cause overestimation of microplastics

#35
Didnt they use for newest studies to detect microplastic in placentas I think only non plastic omitting alternative gloves and material. Can't recall there it was specifically mentioned in a worldclass ARTE docu about microplastics maybe some ARTE Ultras here can recall.

Re: Nitrile and latex gloves may cause overestimation of microplastics

#36
post #23

Earlier quoted context omitted.

I thought that exact thing and opened the comments to see you’d already commented with it. There is a “case files” podcast on it that I found quite good.

This seems to be the Casefile episode about the "Phantom of Heilbronn" https://casefilepodcast.com/case-178-the-woman-without-a-fac...

That's incredible. Though the effect of this will be claims that microplastics don't exist while no one in that case claimed that murders didn't happen. Happy to have learned about an interesting historical oddity either way.

Re: Nitrile and latex gloves may cause overestimation of microplastics

#37

Earlier quoted context omitted.

> Do your own assessment. Yeah, and my primitive home-grown analysis then carries the same weight as those from experts with professional equipment? Oh come on...

Doesn't have to be one or the other. Trust, but verify? Experts make mistakes, professional equipment can be mishandled. Don't take anybodies word, look at the evidence for yourself. This is a very scientific way of thinking. It's only gotten a bad rap on account of people using it to attack others' research and then(crucially) failing to perform their own.

True, trust but verify and start questioning things. Science is now more politicized more than ever by politicians. COVID vaccines are not even tested. I didn't said this. Pfizer and Moderna CEO said this in EU parliament hearing.

Re: Nitrile and latex gloves may cause overestimation of microplastics

#38
post #19

Called it! > To be honest, after reading some of these microplastics papers I'm starting to suspect most of them are bullshit. Plastics are everywhere in a modern lab and rarely do these papers have proper controls, which I suspect would show that there is a baseline level of microplastic contamination in labs that is unavoidable. Petri dishes, pipettes, microplates, EVERYTHING is plastic, packaged in plastic, and cl…

I haven't really read the studies but shouldn't they have negative controls to negate these effects? Wouldn't that let the author's correct for a baseline contamination level in the lab?

That was the difficulty with DNA: how do you make that control if everything is contaminated and minor variations in protocol (like wafting your hands over the samples one too many times) changes the baseline?

It took years to figure out proper methods and many subfields have their own adjusted procedures and sometimes even statistical models. At least with DNA you could denature it very effectively, I’m not sure how they’re going to figure out the contamination issue with microplastics.

Re: Nitrile and latex gloves may cause overestimation of microplastics

#39
post #21
post #19

Earlier quoted context omitted.

I haven't really read the studies but shouldn't they have negative controls to negate these effects? Wouldn't that let the author's correct for a baseline contamination level in the lab?

On the one hand, hundreds or perhaps thousands of studies might be wrong. On the other hand, this one might be wrong. Who's to say?

Not even that! This study doesn't even say contamination is causing overestimation. It says that it's possible.

But as mentioned elsewhere in the thread, everyone knows that it's possible and take measure to mitigate it.

A paper that said those mitigations were insufficient or empirically found not to work would be interesting. A paper saying "you should mitigate this" is... not very interesting.

Re: Nitrile and latex gloves may cause overestimation of microplastics

#40
post #22

So the problem is these particles are literally flying off the gloves of the scientists wearing them to the point it's interfering with the experiment and so... it's less of a problem?

Yes? Most people don’t live their entire lives in a lab wearing nitrile gloves, so there’s an argument to be made that the concentration of microplastics found in that setting is not reflective of everyday life. So, not that microplastics don’t exist, but that they don’t exist to the same degree as in a lab environment.

I wouldn't be surprised if e.g. all these paper-thin synthetic (plastic) disposable parts and fabrics used in labs shed microplastics way more than e.g. synthetic fabrics designed to be survive a machine wash a few dozen times, or upholstery meant to withstand tens of thousands of sitting cycles, nevermind solid plastics (e.g. reusable food containers, furniture surfaces).
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