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Oral microbiome sequencing after taking probiotics

blog.booleanbiotech.com

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Re: Oral microbiome sequencing after taking probiotics

#31
post #28

Earlier quoted context omitted.

As far as I am aware, fiber has infinitely superior research behind it with far more drastic effects. Just take fiber if you're worried about gut health imo.

Yes, feeding good bacteria is the best bet. Probiotics are transient, they don’t colonize.

How do they get there initially?

Re: Oral microbiome sequencing after taking probiotics

#32

Earlier quoted context omitted.

When I asked my doctor about which kinds of probiotics are most effective she specifically mentioned refrigerated vs non-refrigerated is not a way to identify quality or effectiveness unless you know the specific strain(s) needed cannot be made shelf-stable. This lined up with asking my endodontist after they prescribed some antibiotics for a tooth infection. They did warn that the use by dates are a bit bull, not to…

Also frankly, doctor =/= expert on probiotics. None of their training really addresses that and while they might be more qualified to read research than random layman I would not in general ascribe authority to what a random practitioner has to say about probiotics. Frankly, the research on probiotics is still very much in its infancy and a LOT remains to be figured out.

> doctor =/= expert on probiotics

Medical microbiologists would love to have a word with you. Medicine and medicine-adjacent disciplines each develop institutional knowledge that percolates from each specialized discipline.

> …the research on probiotics is still very much in its infancy and a LOT remains to be figured out.

I’m curious who you think does the research. It’s certainly not Bubba from down the creek.

Re: Oral microbiome sequencing after taking probiotics

#33

>However, there is some light evidence that the variation I see is not just intra-day variation. Specifically, there are several species that stay consistent in frequency across all samples: e.g., Neisseria subflava, Streptococcus viridans, Streptococcus oralis. Disagree. You can not make that claim without sequencing your mouth's microbiome in the absence of probiotics for a month as well (and, really, many more tha…

Well, they did say "light evidence" rather than "proof".

Re: Oral microbiome sequencing after taking probiotics

#34

PSA: If a probiotic is on the shelf, not in a cooler, it's probably not worth buying. The best companies certify the count of organisms at time of manufacture , but no counts are guaranteed at the shelf. Probiotics are living organisms and ought to be refrigerated for max lifespan. You can get refrigerated probiotic supps at a place like Whole Foods. Source: I used to work in the industry.

When I asked my doctor about which kinds of probiotics are most effective she specifically mentioned refrigerated vs non-refrigerated is not a way to identify quality or effectiveness unless you know the specific strain(s) needed cannot be made shelf-stable. This lined up with asking my endodontist after they prescribed some antibiotics for a tooth infection. They did warn that the use by dates are a bit bull, not to…

“ and not to try to keep even the shelf-stable ones above room temperature.” - hate to ask, but night brain kicked in - could I trouble you to give me an alternative way of phrasing this? I keep parsing it as “don’t even bother trying to keep probiotics warm”

Re: Oral microbiome sequencing after taking probiotics

#35

PSA: If a probiotic is on the shelf, not in a cooler, it's probably not worth buying. The best companies certify the count of organisms at time of manufacture , but no counts are guaranteed at the shelf. Probiotics are living organisms and ought to be refrigerated for max lifespan. You can get refrigerated probiotic supps at a place like Whole Foods. Source: I used to work in the industry.

They sell a yogurt starter that is purportedly Salivarius and ruteirii culture over at Beazos' Clubhouse. I haven't had it tested but it's way easier than the usual Bulgarian microbes. Assuming the cultures are as labeled, I have to imagine eating live culture yogurt is more likely to propogate than loszenges made in a factory though.

Re: Oral microbiome sequencing after taking probiotics

#36

Earlier quoted context omitted.

When I asked my doctor about which kinds of probiotics are most effective she specifically mentioned refrigerated vs non-refrigerated is not a way to identify quality or effectiveness unless you know the specific strain(s) needed cannot be made shelf-stable. This lined up with asking my endodontist after they prescribed some antibiotics for a tooth infection. They did warn that the use by dates are a bit bull, not to…

“ and not to try to keep even the shelf-stable ones above room temperature.” - hate to ask, but night brain kicked in - could I trouble you to give me an alternative way of phrasing this? I keep parsing it as “don’t even bother trying to keep probiotics warm”

"Even the ones that can be kept outside a fridge shouldn't be kept above room temperature".

Re: Oral microbiome sequencing after taking probiotics

#37

PSA: If a probiotic is on the shelf, not in a cooler, it's probably not worth buying. The best companies certify the count of organisms at time of manufacture , but no counts are guaranteed at the shelf. Probiotics are living organisms and ought to be refrigerated for max lifespan. You can get refrigerated probiotic supps at a place like Whole Foods. Source: I used to work in the industry.

When I asked my doctor about which kinds of probiotics are most effective she specifically mentioned refrigerated vs non-refrigerated is not a way to identify quality or effectiveness unless you know the specific strain(s) needed cannot be made shelf-stable. This lined up with asking my endodontist after they prescribed some antibiotics for a tooth infection. They did warn that the use by dates are a bit bull, not to…

> maybe my doctor was just wrong

Yes, doctors are similar to mechanics or any other trade, in that some simply suck.

Some got Ds.

Re: Oral microbiome sequencing after taking probiotics

#38

Earlier quoted context omitted.

Yes

The Internet almost universally disagrees, based on a quick search: https://seed.com/cultured/probiotics-refrigeration-storage-g...

Why do you think they’re here, sharing the info with you?

If you could just Google it up, not nearly as interesting to HN.

Re: Oral microbiome sequencing after taking probiotics

#39

Only tangentially relevant, but I’ve dealt with mouth and gut microbiome issues my whole life, the latter exacerbated by a strong antibiotic I had to go on in mid 2017 for a super resistant staph infection. L Reuteri supplementation and “L Reuteri yogurt” was one of those alternative methods I read about (though I’m skeptical that reuteri is the dominant strain in this “yogurt”) Doctors don’t really care to look at t…

> Doctors don’t really care to look at these kinds of issues

Perhaps for good reasons?

The science is messy, there are few proven interventions and every woowoo worrywart will be pestering their doctor. Your doctor is in an unenviable position.

With doctors in New Zealand, my one trick is to find good specialists and pay them privately.

I believe that a GP only helps point you in the right direction. Our public health system is mostly too overloaded to help (unless you have a critical problem and your GP helps you get in a queue).

Not sure what helps in other countries.

But I 100% agree that you need to take responsibility for healing yourself. Only you have the motivation, and the context and experience to judge your own problems -- however one needs to take care not to get caught in irrelevant or misleading deadends (especially when mislead by corporations or alternative woowoo freaks).

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