Can we ever imagine dna sequencing been done like a remoter sensor? E.g. automated sampling for e(environmental) dna,in-situ sequencing (single and multi species targeting), results transmitted back wirelessly. A biodiversity IoT system?
Nanopore MinION – $1k solid-state DNA sequencers
31–40 of 111 posts
Re: Nanopore MinION – $1k solid-state DNA sequencers
#32Can we ever imagine dna sequencing been done like a remoter sensor? E.g. automated sampling for e(environmental) dna,in-situ sequencing (single and multi species targeting), results transmitted back wirelessly. A biodiversity IoT system?
All the technology required already exists. You'd just need a compelling enough application.
Re: Nanopore MinION – $1k solid-state DNA sequencers
#33I need one of these to identify the neighbor that lets their dog fertilize my lawn. At least .. I hope it's a dog.
A $100 wildlife camera may solve that problem for you.
Re: Nanopore MinION – $1k solid-state DNA sequencers
#34Wow. Can I do whole-genome sequencing with this? Is this a similar product/result as buying whole genome sequencing from an established company for the $500 to $800 they charge these days?
The minion is a device you own. You don't need to ship the samples (which can be a problem, imagine ebola samples). You wait a maximum of 2 days for most of the results.
Re: Nanopore MinION – $1k solid-state DNA sequencers
#35I just ran a couple of flowcells last week. I've ran 8 total so far now. My impression is that it can be very inconsistent depending on the quality of the flowcell that they send you and your DNA prep. Great for smallish genomes (bacteria), not enough throughput/quality for large eukaryotic genomes (1gb+) unless you got money to burn.
It’s also comparatively expensive compared to other platforms (you can get a full human genome sequenced at high coverage for between 1000 and 3000 USD).
The error rate is stupidly high (somewhere between 10 and 20%) compared to Illumina or Ion Torrent who give error rates far less than 1%.
It can give very long reads, which are useful in some niche applications. But it’s been massively over-hyped (and over capitalized).
The neat thing is that it’s very small. But that isn’t really compelling given the very low accuracy.
Re: Nanopore MinION – $1k solid-state DNA sequencers
#36So what’s a whole human genome sequence cost? That’s the usual metric machines are listed by.
I believe Nanopore Technologies is about to announce that a $1000 Human genome is possible with the PromethION, a big brother of the MinION.
Re: Nanopore MinION – $1k solid-state DNA sequencers
#37How much are the flow cells?
Ah. ~$500 - $1000 each. That seems like an expensive consumable, but I have no basis for comparison. Is this a lot? https://store.nanoporetech.com/flowcells.html
It's the major cost of sequencing with this system. Apparently, overall, the cost is a bit higher than with the current industry leader.
Apparently the cost is competitive for what it does.
Re: Nanopore MinION – $1k solid-state DNA sequencers
#38How far away we are from a 100$ genome? I've heard that the cost/performance development rate is 'super-Moorean'.
Here's the Sequencing & Moore's Law plot that I'm tired of seeing in every genome sequencing presentation ever. https://wi-images.condecdn.net/image/W5YlBgPjX36/crop/1620 Since 2013, Illumina's brought the price of a genome to about $1k with the HiSeq X, which essentially scales up their existing tech. No idea what the next few years hold, but I'm excited to see insurance companies beginning to cover genome sequencin…
The question is also what exactly you get. Illumina's technology gives you very short pieces of DNA. I'd call it confetti sequencing. Pacbio and Nanopore both go for longer sequences. This is important when trying to assemble the pieces to a bigger picture.
Re: Nanopore MinION – $1k solid-state DNA sequencers
#39I came across this at a MongoDB conference last year, and thought the demos / videos looked bloody cool.
Here's some more detail on the tech works: https://www.mongodb.com/blog/post/oxford-nanopore-technologi...
Re: Nanopore MinION – $1k solid-state DNA sequencers
#40I came across this at a MongoDB conference last year, and thought the demos / videos looked bloody cool. Here's some more detail on the tech works: https://www.mongodb.com/blog/post/oxford-nanopore-technologi...
That’s extremely interesting, thank you for posting the link.