Craig Venter, the first genome sequenced and first promoter of shotgun sequencing, disses CRISPR. He claims its not reliable enough. Nor can you prevent from altering unexpected parts of the genome.
I dont know how much is not-invented-here and how accurate he is.
CRISPR eliminates HIV-1 infection in live animals
251–260 of 267 posts
Re: CRISPR eliminates HIV-1 infection in live animals
#252Earlier quoted context omitted.
"More practically, HIV has such a high mutation rate, that it's likely very difficult to target every HIV sequence with a sequence-specific Cas9 therapy. If the Cas9 guide sequence is too generic it'll take out stuff besides HIV (stuff you need). " Unless I'm grossly misunderstanding how CRISPR works, there's no conceptual reason why you couldn't target multiple sequences at the same time , with a cocktail method. Th…
I think ∼3E−5 per base per replication. Might be a more useful number [1]. If you use 10mers, that only gives you 1048576. I'd be almost certain that >90% of those sequences also exist in the human genome. So your target isn't specific enough (take out stuff you need as the parent suggested). So you need to use a longer sequence, perhaps 25bp. Maybe there's a stable region or set of regions you can target (in which c…
"Maybe there's a stable region or set of regions you can target (in which case the high overall mutation rate doesn't matter). Or a cocktail of sequences, specific to the global HIV population (I doubt this, HIV mutates more in a single individual than Flu does in the global population)."
Hmm... I would bet that there is a cocktail of sequences such that if they are not conserved, the virus effectively becomes no longer HIV (no longer infectious, no longer capable of producing symptoms...).
HIV is obviously not a human being, right? Find every sequence where it differs, target them all. :-)
Re: CRISPR eliminates HIV-1 infection in live animals
#253Earlier quoted context omitted.
What your comment (and followup) shows is that you think some other people's behaviour makes them deserve HIV. That's an utterly intolerable position, and especially so in this community. Nobody deserves HIV.
What about the degenerate subculture of "bug chasers"? Aren't they literally asking for it?
We've banned this account.
Re: CRISPR eliminates HIV-1 infection in live animals
#254I am Cristina Pereira,I contracted HIV in 2009 I was told by my doctor that there's no possible cure for HIV. I started taking my ARV's, My CD4 is 77. I saw a website of Dr Abai also i saw a lot of testimonials about him on how he uses herbal medicine to cure HIV. i contacted him and told him my problems, he sent me the herbal medicine and i took it for 7 days after then i went for a check up and i was Negative. the medicine has no side effect, there's no special diet when taking the medicine visit Dr Abai website: http://drabaiherbalcure.webs.com and contact him through his WHATS-APP NUMBER +2348156769001, or contact him via email:drabaiherbalcure@yahoo.com
Re: CRISPR eliminates HIV-1 infection in live animals
#255I am Cristina Pereira,I contracted HIV in 2009 I was told by my doctor that there's no possible cure for HIV. I started taking my ARV's, My CD4 is 77. I saw a website of Dr Abai also i saw a lot of testimonials about him on how he uses herbal medicine to cure HIV. i contacted him and told him my problems, he sent me the herbal medicine and i took it for 7 days after then i went for a check up and i was Negative. the medicine has no side effect, there's no special diet when taking the medicine visit Dr Abai website: http://drabaiherbalcure.webs.com and contact him through his WHATS-APP NUMBER +2348156769001, or contact him via email:drabaiherbalcure@yahoo.com
Re: CRISPR eliminates HIV-1 infection in live animals
#256I am Cristina Pereira,I contracted HIV in 2009 I was told by my doctor that there's no possible cure for HIV. I started taking my ARV's, My CD4 is 77. I saw a website of Dr Abai also i saw a lot of testimonials about him on how he uses herbal medicine to cure HIV. i contacted him and told him my problems, he sent me the herbal medicine and i took it for 7 days after then i went for a check up and i was Negative. the medicine has no side effect, there's no special diet when taking the medicine visit Dr Abai website: http://drabaiherbalcure.webs.com and contact him through his WHATS-APP NUMBER +2348156769001, or contact him via email:drabaiherbalcure@yahoo.com
Re: CRISPR eliminates HIV-1 infection in live animals
#257I am Cristina Pereira,I contracted HIV in 2009 I was told by my doctor that there's no possible cure for HIV. I started taking my ARV's, My CD4 is 77. I saw a website of Dr Abai also i saw a lot of testimonials about him on how he uses herbal medicine to cure HIV. i contacted him and told him my problems, he sent me the herbal medicine and i took it for 7 days after then i went for a check up and i was Negative. the medicine has no side effect, there's no special diet when taking the medicine visit Dr Abai website: http://drabaiherbalcure.webs.com
Re: CRISPR eliminates HIV-1 infection in live animals
#258Earlier quoted context omitted.
> You can spend hundreds of millions of dollars before you even get to the point where you can ask the FDA if you can start human trials. What's a rough breakdown of costs? Salaries certainly don't seem to be the dominant factor. Is it lab equipment & facilities?
I'm not a chemist, but here's how I understand it to work. It's all trial and error. You start with some model about how your target disease works. Perhaps, for the sake of argument, your model is that disease Q is caused by a deficit of protein N. Protein N is broken down by enzyme F, so obviously if you found a drug that suppressed enzyme F, you could cure disease Q. Now all you have to do is try every chemical you…
Thanks, that's great.
Re: CRISPR eliminates HIV-1 infection in live animals
#259Earlier quoted context omitted.
Seems XKCD was on point this week: https://www.xkcd.com/1831/
To be fair, a lot of breakthroughs happen by people outside the field in question applying new ways of thinking to that field. Sure, these people can be kind of annoying, but I think we lose more than we gain by discouraging cross-pollination between fields of science.
Douglas Crockford should learn from you and use the term cross-pollination instead of promiscuity. That would have saved him a lot of trouble
Re: CRISPR eliminates HIV-1 infection in live animals
#260Earlier quoted context omitted.
Seems XKCD was on point this week: https://www.xkcd.com/1831/
To be fair, a lot of breakthroughs happen by people outside the field in question applying new ways of thinking to that field. Sure, these people can be kind of annoying, but I think we lose more than we gain by discouraging cross-pollination between fields of science.
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