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CRISPR eliminates HIV-1 infection in live animals

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Re: CRISPR eliminates HIV-1 infection in live animals

#231

Post by virusduck »

lr4444lr wrote: post #218
alevskaya wrote: post #207

Earlier quoted context omitted.

Very over-simplified: You have a random library of many billions of cells each making a single unique antibody that was created via random combinatorial genetic shuffling early on. The ones that accidentally bind to your own natural proteins are filtered out by killing them before they leave the bone marrow, so the circulating cells remaining form a library that could only bind -foreign- proteins. When one of these f…

Is there concern that those long-lived antibody clones become pathological themselves?

Yes and no. Yes in that some of the many billions of combinations of antibody genes can recognize self proteins, which is a problem. However, during maturation of B cells, the immune system has a mechanism for killing of those B cells which would produce anti-self antibodies before they mature. There are times when this fails to work--think Grave's Disease, many forms of lupus, alopecia, etc.

I think in the sense you are asking, though, is that any long-lived plasma cell or memory B cell that is active will probably not change to the extent that they would attack self. I don't know off the top of my head if there are examples of this, but I can't think of any.

Re: CRISPR eliminates HIV-1 infection in live animals

#232

Post by aphextron »

Pyxl101 wrote: post #195
alevskaya wrote: post #190

Earlier quoted context omitted.

I fully support basic science pursuing crazy ideas. I think this is a very interesting piece of basic science, it's just at an incredibly speculative stage that's unsuitable for clinical investment. Efficient delivery of novel proteins into a cell by genetic methods, nanoparticles, or direct transduction is -the- challenge for a lot of novel ideas. Massive effort is ongoing to find breakthroughs here. The proteins in…

This is fascinating. How in the world does the body "raise antibodies" that are effective against arbitrary proteins it hasn't seen before? How does this get "remembered" and how does the memory get communicated through the body? If there's an ELI5 (or, ELI-college-101) I'd be interested to read it.

> How does this get "remembered" and how does the memory get communicated through the body?

https://en.wikipedia.org/wiki/RNA

Re: CRISPR eliminates HIV-1 infection in live animals

#233

Post by sctb »

p_zakharov wrote: post #194

I am very much in favor of genetic engineering and gene therapy in general, though I actually have mixed feelings about this. The old song "HIV+" by one-man industrial act Suicide Commando described the virus as "God's reprisal". Metaphorically, at least, this appears frequently to be the case; there is a downside to curing HIV. But I expect that success—whether or not this particular finding will carry over into a v…

This kind of ideological flamebait (regardless of its particular ideology) doesn't belong on Hacker News. Please don't post like this here.

https://news.ycombinator.com/newsguidelines.html

Re: CRISPR eliminates HIV-1 infection in live animals

#234

Post by dmix »

Animats wrote: post #107
dmix wrote: post #53

Earlier quoted context omitted.

This is the type of innovation we exchange for a sense of absolute security in having highly-vetted decade-long bio R&D and extensive backlogs at the FDA for approvals to sell products on the market. It's go big or go home because the regulatory risk/timeline is so extensive. Most of these things die quietly or never get started so we never really see the true costs of what it takes to push the pharma world forward.…

No, this hasn't gotten anywhere near human testing yet. The FDA isn't involved.

Obviously it will be in the future, which deters any small scale seed investment...

Re: CRISPR eliminates HIV-1 infection in live animals

#235

Post by p_zakharov »

sctb wrote: post #233
p_zakharov wrote: post #194

I am very much in favor of genetic engineering and gene therapy in general, though I actually have mixed feelings about this. The old song "HIV+" by one-man industrial act Suicide Commando described the virus as "God's reprisal". Metaphorically, at least, this appears frequently to be the case; there is a downside to curing HIV. But I expect that success—whether or not this particular finding will carry over into a v…

This kind of ideological flamebait (regardless of its particular ideology) doesn't belong on Hacker News. Please don't post like this here. https://news.ycombinator.com/newsguidelines.html

I find it difficult to adhere to the meaning of guidelines like this, because I don't know exactly what they intend. For example, I would say what I did in a face-to-face conversation, and I don't think I'm being gratuitously negative.

Re: CRISPR eliminates HIV-1 infection in live animals

#236

Post by mandude »

maverick_iceman wrote: post #168
mandude wrote: post #2

Gene therapy (and CRISPR) is ultimately going to eliminate most if not every ailment/issue/genetic problem that we have, including aging and eye problems. Very thankful we are reaching the point where we can go into our cells and fix ourselves.

Evidence?

https://www.researchgate.net/publication/315775073_In_Vivo_E...

Re: CRISPR eliminates HIV-1 infection in live animals

#237

Post by Blahah »

Blahah wrote: post #196
p_zakharov wrote: post #194

I am very much in favor of genetic engineering and gene therapy in general, though I actually have mixed feelings about this. The old song "HIV+" by one-man industrial act Suicide Commando described the virus as "God's reprisal". Metaphorically, at least, this appears frequently to be the case; there is a downside to curing HIV. But I expect that success—whether or not this particular finding will carry over into a v…

What is the downside to curing HIV?

replying to myself to say that parent replied:

> Degenerate behavior can't be punished with AIDS anymore.

I think that's important context for their original post, which is why I'm posting it although it was flagged and downvoted to death.

Re: CRISPR eliminates HIV-1 infection in live animals

#238

Post by [deleted] »

Blahah wrote: post #196
p_zakharov wrote: post #194

I am very much in favor of genetic engineering and gene therapy in general, though I actually have mixed feelings about this. The old song "HIV+" by one-man industrial act Suicide Commando described the virus as "God's reprisal". Metaphorically, at least, this appears frequently to be the case; there is a downside to curing HIV. But I expect that success—whether or not this particular finding will carry over into a v…

What is the downside to curing HIV?

[deleted]

Re: CRISPR eliminates HIV-1 infection in live animals

#239

Post by Blahah »

p_zakharov wrote: post #235
sctb wrote: post #233

Earlier quoted context omitted.

This kind of ideological flamebait (regardless of its particular ideology) doesn't belong on Hacker News. Please don't post like this here. https://news.ycombinator.com/newsguidelines.html

I find it difficult to adhere to the meaning of guidelines like this, because I don't know exactly what they intend. For example, I would say what I did in a face-to-face conversation, and I don't think I'm being gratuitously negative.

What your comment (and followup) shows is that you think some other people's behaviour makes them deserve HIV. That's an utterly intolerable position, and especially so in this community. Nobody deserves HIV.

Re: CRISPR eliminates HIV-1 infection in live animals

#240

Post by sctb »

p_zakharov wrote: post #235
sctb wrote: post #233

Earlier quoted context omitted.

This kind of ideological flamebait (regardless of its particular ideology) doesn't belong on Hacker News. Please don't post like this here. https://news.ycombinator.com/newsguidelines.html

I find it difficult to adhere to the meaning of guidelines like this, because I don't know exactly what they intend. For example, I would say what I did in a face-to-face conversation, and I don't think I'm being gratuitously negative.

This case is well covered by the guideline that says:

> Please avoid introducing classic flamewar topics unless you have something genuinely new to say about them.

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