211–220 of 700 posts
Low-level stuff & Linux: RISC-V, Vulkan, Wayland, Sway WM, Wireguard, Zig. Web or high-level stuff: deno, Svelte, Vue, Webassembly, WebGPU, Flutter.
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> It's an ISA that looks set to be adopted in a pretty wide range of applications, web browsers, sandboxed and cross platform applications, embedded (into other programs) scripting, cryptocurrencies, Imagine if the crowd didn't fall for the HODL hypers and called these things cryptolotteries or something like that -- they are a betting game after all -- how ridiculous would it look to include them in every discussion…
What are you adding to the discussion? This is a technical forum, the least you could do is comment on the use of Web Assembly in Ethereum or maybe anything of substance. There's a bunch of technically interesting topics to bring up but somehow I doubt you know anything about them.
1. Functional strictly typed programming patterns. It's hard to say if functional languages themselves will get adoption, but we definitely see functional patterns being used more and more in languages like Javascript, and being pushed in things like React/Redux. 2. Graphql/service-based architectures
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We're a nanopore sequencing shop. Couldn't agree with you more that nanopore generally is the future, not just for sequencing. Can't wait for ONT's solid state nanopore flow cells - you may get flowcells that go 2/3 times as long then. But then for most things .. the problem is the prep, not just because the whole portability thing goes for a toss. Yay, great the sequencer is the size of a USB drive, but the rest of…
The key is to do barcoding at the PCR amplification step. That way, you can get away with barcoding hundreds of samples in a single tube. Really the prep screws it any other way. Is ONT coming out with solid state? Got a reference?
I'm pretty sure they will come out with it. The protein nanopores were the first wave of nanopore research - its tried tested and stable now so they stick with it.
A few years after they launched, the first solid state nanopores were being demonstrated in the lab. Commercialising solid state nanopores seems to be easier if anything than protein nanopores because they slot right into silicon fabrication.
On PCR/barcoding .. Yeah, thats right - do it right in the PCR step .. sometimes we avoid it, if we are not yet sure about the protocol. I think what I meant to say is that the full promise of nanopore sequencing for me is achieved only when you can skip having to amplify/multiplex/barcode - just extract dna, wash, add sequencing adaptors and go - for almost anything.
I think the way they are talked about people generally come in expecting that TODAY .. they think they can literally stick a single sample with no prep and get 1gb of sequencing done for 10$ in an hour. I've seen quite a lot of that. (even from people with PhD's :) )
So yeah its more that the minute you go PCR, you're in for a minimum 20$ per sample, often its the highest cost line item in your whole process.
If you're doing things like 16S metagenomics, you get sequencing at 2-3$ and prep at 10 times that, starts to feel "wrong" after a while, if you get what I mean.
Why're trying everything we can to make sure we're running at full capacity so that we can always give low prices even if its single samples.
Also seeing if we can reduce prep cost with microfluidics/mems - they have voltrax for this, but there are a few other vendors in the market. That has the positive knock-on effect of also reducing labour cost.
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What are you adding to the discussion? This is a technical forum, the least you could do is comment on the use of Web Assembly in Ethereum or maybe anything of substance. There's a bunch of technically interesting topics to bring up but somehow I doubt you know anything about them.
I speak up against cryptocurrency because it's a cancer. It's a hype adding to climate change without any real world use case whatsoever.
If you read about the Baseline protocol (EY, Microsoft, SAP etc building neutral interconnections between consortiums), ENS/IPFS, or digital identity systems you might find something that interests you and is more relevant than the mindless hodl ancaps. It's actually a pretty exciting field to be in as a computer scientist with almost no end of boundary pushing experiments and cryptographic primitives to play with and build on top of.
Ethereum becoming the value settlement layer of Web 3.0 [1]. Stablecoins have proven to be the killer dApp and there are ~$10B in circulation currently [2]. [1] https://ethereum.org/ [2] https://stablecoinstats.com/