For big business? Nil. Big business doesn't need 3rd party cloud services other than as something to sell to offset their own infrastructure investment.
As to who these sanctions are biting. Sanctions always bite someone, but rarely do they hurt leaders. I think often they can help extend a regime's grip because it plays into propaganda.
Also, if sanctions were imposed generally for dicking about in other countries on dubious pretexts, then nobody would be trading with the UK or US right now. Glass houses and all that.
edit - if you want to influence Russia at the moment, either you please Putin, or work out how to get rid of him. Pissing him off won't help. He is a very experienced KGB guy who has oversaw the period of Russia returning from a brink of near dissolution to a state that is at least functioning, and people put up with a lot of crap to have something that functions.
There are parallels with China here in that the insanity of the Mao years can be used to justify putting up with a lack of political freedom today, on the basis that at least the current leaders aren't completely crazy. In Russia, people compare Putin with Yeltsin and think that things could be a lot worse as at least Putin can find his arse with both hands and isn't pissed all the time. Is the political version of New Coke.
To that end, any sanctions that do not actually destabilise Russia are probably counter productive and any that do destabilise Russia are just plain dangerous.
The question we really need to answer quickly is what does Putin actually want. If he is a Napoleon in waiting, we should try and depose him rather than pussyfooting around, whereas if he is merely a bit of a despot but not intent on conquest then it would be better to keep him happy and wait.
The Crimea doesn't really tell us much on that as it is where the Black Sea fleet has been kept since Potemkin's time, and so can be argued as a defensive as much as an offensive move. I am somewhat cheered by Putin's apparent lukewarm views towards the separatist movements in eastern Ukraine, though this could be a complete front.
I was convinced that he was going to try and force and hold a land corridor to the Crimea and the fact that this has not been done (yet) might indicate that Putin is not so much intent on expansion but is just keeping the whole thing warm to keep folk busy, much like China does with North Korea. If that is true, then hopefully he has had a bit of an 'Oh Fuck' moment with the airliner going down.