Earlier quoted context omitted.
I was briefly involved in Single Molecule Biophysics, where microscopes push the limits of what you can do with visible light in order to understand protein behaviour, where I heard the often repeated warning: "the microscope is an experiment itself". This was born out of the incredibly fancy and poorly understood microscope, which was experimental, and it was no guarantee that you were measuring the protein and not…
> and it was no guarantee that you were measuring the protein and not some weird light physics effect! My thesis was in fluorescent bio physics at the nano scale (STED). We literally had a PI effect. Normally, the 'PI effect' is that when the professor comes in, the experiment stops working, mostly due to Murphy's law. With our case, it was because the extra body heat caused the mirrors to expand and drift out of pla…
When designing sensitive instruments we had to use almost a paranoid attention to detail.