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American chestnut tree in Centreville is the 'holy grail' for conservationists

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11–20 of 157 posts

Re: American chestnut tree in Centreville is the 'holy grail' for conservationists

#11
If this tree had a secret to cultivating a resistant strain that would be very awesome.

Is there a good app which lets you input your zip code, maybe also sun exposure and soil conditions, how many feet of width and height you have available, and then where it will spit out a list of well-suited tree specimens with some nice pictures of each?

Re: American chestnut tree in Centreville is the 'holy grail' for conservationists

#12
post #5

Saw this really amazing video[0] the other day about American Chestnut Foundation's breeding program in Virginia to revive the American chestnut Basically they hybridized it with Chinese/Japanese chestnuts (which are blight resistant). Then they take the offspring, test for blight resistance and rebreed those back with purebred American chestnuts. Then they repeat the process. Take those offspring, select for blight…

Saw that video recently as well. What's remarkable about this find is the size of the specimen, and that it's confirmed to be 100% American Chestnut. Hopefully with the hybrid approach, this find, and further breeding/propagation work, we can see a large scale return of this incredible species.

Re: American chestnut tree in Centreville is the 'holy grail' for conservationists

#13
> There used to be about 4 million American chestnut trees in eastern U.S. forests, until chestnut blight (Cryphonectria parasitica) arrived in New York via infected plants and spread rapidly, nearly wiping out the majestic trees

Fwiw this is wrong -- it used to be 4 billion.

Re: American chestnut tree in Centreville is the 'holy grail' for conservationists

#14
post #8
post #5

Saw this really amazing video[0] the other day about American Chestnut Foundation's breeding program in Virginia to revive the American chestnut Basically they hybridized it with Chinese/Japanese chestnuts (which are blight resistant). Then they take the offspring, test for blight resistance and rebreed those back with purebred American chestnuts. Then they repeat the process. Take those offspring, select for blight…

Why don't they use agrobacterium transfection, gene guns, or molecular techniques to directly transfer the resistance genes over? Do we not know what the genes themselves are? I'm not criticizing their technique! Nor do I think I know better. This is amazing and valuable work, and I'd love to know more.

IIRC the breeding program predates those tools.

Re: American chestnut tree in Centreville is the 'holy grail' for conservationists

#15
post #8
post #5

Saw this really amazing video[0] the other day about American Chestnut Foundation's breeding program in Virginia to revive the American chestnut Basically they hybridized it with Chinese/Japanese chestnuts (which are blight resistant). Then they take the offspring, test for blight resistance and rebreed those back with purebred American chestnuts. Then they repeat the process. Take those offspring, select for blight…

Why don't they use agrobacterium transfection, gene guns, or molecular techniques to directly transfer the resistance genes over? Do we not know what the genes themselves are? I'm not criticizing their technique! Nor do I think I know better. This is amazing and valuable work, and I'd love to know more.

Low-tech, non flash solution that delivers.

Re: American chestnut tree in Centreville is the 'holy grail' for conservationists

#16
post #8
post #5

Saw this really amazing video[0] the other day about American Chestnut Foundation's breeding program in Virginia to revive the American chestnut Basically they hybridized it with Chinese/Japanese chestnuts (which are blight resistant). Then they take the offspring, test for blight resistance and rebreed those back with purebred American chestnuts. Then they repeat the process. Take those offspring, select for blight…

Why don't they use agrobacterium transfection, gene guns, or molecular techniques to directly transfer the resistance genes over? Do we not know what the genes themselves are? I'm not criticizing their technique! Nor do I think I know better. This is amazing and valuable work, and I'd love to know more.

Good question. I'd imagine something like blight resistance would probably not be a simple case of a few specific genes we can just switch over.

It could also just be technology. This breeding program has been around for 50 years or so and CRISPR only really took off around 2011. In addition, the original CRISPR-Ca9 caused a lot of unintended changes in DNA and it's really critical that the new trees are morphologically equivalent to the original trees in order for the birds, insects, and animals that depended on them to resume their interactions. Newer technologies like CRISPR-Nickase are much more precise but also very recently developed

Re: American chestnut tree in Centreville is the 'holy grail' for conservationists

#17
post #8
post #5

Saw this really amazing video[0] the other day about American Chestnut Foundation's breeding program in Virginia to revive the American chestnut Basically they hybridized it with Chinese/Japanese chestnuts (which are blight resistant). Then they take the offspring, test for blight resistance and rebreed those back with purebred American chestnuts. Then they repeat the process. Take those offspring, select for blight…

Why don't they use agrobacterium transfection, gene guns, or molecular techniques to directly transfer the resistance genes over? Do we not know what the genes themselves are? I'm not criticizing their technique! Nor do I think I know better. This is amazing and valuable work, and I'd love to know more.

If I recall, someone has jammed a wheat gene into a few of them in just that manner.

It's a tricky business, because chestnut had very particular properties, as wood, that made it quite valuable, and any gene interference, well ... who knows how it will change the resultant wood?

Re: American chestnut tree in Centreville is the 'holy grail' for conservationists

#18
post #11

If this tree had a secret to cultivating a resistant strain that would be very awesome. Is there a good app which lets you input your zip code, maybe also sun exposure and soil conditions, how many feet of width and height you have available, and then where it will spit out a list of well-suited tree specimens with some nice pictures of each?

Why stop at trees? I imagine you could do an alternate reality app for landscaping that shows you what your landscape "could look like". Including flower beds, ground cover alternatives to grass, etc.

Re: American chestnut tree in Centreville is the 'holy grail' for conservationists

#19
post #8
post #5

Saw this really amazing video[0] the other day about American Chestnut Foundation's breeding program in Virginia to revive the American chestnut Basically they hybridized it with Chinese/Japanese chestnuts (which are blight resistant). Then they take the offspring, test for blight resistance and rebreed those back with purebred American chestnuts. Then they repeat the process. Take those offspring, select for blight…

Why don't they use agrobacterium transfection, gene guns, or molecular techniques to directly transfer the resistance genes over? Do we not know what the genes themselves are? I'm not criticizing their technique! Nor do I think I know better. This is amazing and valuable work, and I'd love to know more.

Do we not know ....

Re: American chestnut tree in Centreville is the 'holy grail' for conservationists

#20
post #5

Saw this really amazing video[0] the other day about American Chestnut Foundation's breeding program in Virginia to revive the American chestnut Basically they hybridized it with Chinese/Japanese chestnuts (which are blight resistant). Then they take the offspring, test for blight resistance and rebreed those back with purebred American chestnuts. Then they repeat the process. Take those offspring, select for blight…

The chestnut project is from the Commonwealth of Virginia, not West Virginia.
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