11–20 of 179 posts
This is very cool. Are there by chance any associated projects that could evolve into something like 23andme but remain entirely within a private network meaning that the data is entirely in the hands of the individual?
Sounds like a fediverse project?
This is very cool. Are there by chance any associated projects that could evolve into something like 23andme but remain entirely within a private network meaning that the data is entirely in the hands of the individual?
A used laboratory grade NGS system can be had for less than 10K https://www.ebay.com/itm/265148387179 Nanopore is still not quite ready yet for precise and high accuracy sequencing. Give it another five years.
Earlier quoted context omitted.
A used laboratory grade NGS system can be had for less than 10K https://www.ebay.com/itm/265148387179 Nanopore is still not quite ready yet for precise and high accuracy sequencing. Give it another five years.
wow i didn’t know they were that “cheap” now. i used to work for a major competitor to the sequencer you linked, the SOLiD. and i feel like nanopore is the VR of dna sequencing. it’s always just another few years off.
Is this also true for nanopores in protein sequencing? This HN comment from a few weeks back [1] pointed out recent progress but perhaps the tech is still not quite there.
This is very cool. Are there by chance any associated projects that could evolve into something like 23andme but remain entirely within a private network meaning that the data is entirely in the hands of the individual?
yes. if you wanted to annotate your genome you could “easily” do it on your brand new macbook (this is ram intensive, you probably need 32G). you’d need a reference genome, like https://www.nist.gov/programs-projects/genome-bottle then you’d need a program like bwa http://bio-bwa.sourceforge.net/ to map your data. then use https://samtools.github.io/bcftools/howtos/variant-calling.h... or something else to produce va…
Earlier quoted context omitted.
yes. if you wanted to annotate your genome you could “easily” do it on your brand new macbook (this is ram intensive, you probably need 32G). you’d need a reference genome, like https://www.nist.gov/programs-projects/genome-bottle then you’d need a program like bwa http://bio-bwa.sourceforge.net/ to map your data. then use https://samtools.github.io/bcftools/howtos/variant-calling.h... or something else to produce va…
I'm unclear from this what kind of equipment you need to extract and analyze the material?
Earlier quoted context omitted.
Sounds like a fediverse project?
Oh God, I would not want a distributed group of actors with limited trust to sequence my DNA. Maybe it's a project for close group of friends that would be interested?
But this could enable things like finding relatives which is what I got out of the comment about 23andme. Instead of all the data being centralized, storage and comparison could be distributed
This is very cool. Are there by chance any associated projects that could evolve into something like 23andme but remain entirely within a private network meaning that the data is entirely in the hands of the individual?
A used laboratory grade NGS system can be had for less than 10K https://www.ebay.com/itm/265148387179 Nanopore is still not quite ready yet for precise and high accuracy sequencing. Give it another five years.
Earlier quoted context omitted.
A used laboratory grade NGS system can be had for less than 10K https://www.ebay.com/itm/265148387179 Nanopore is still not quite ready yet for precise and high accuracy sequencing. Give it another five years.
I work in a dry lab but I'm pretty sure you need a lot of expensive chemicals to actually make one of these work, yeah?
Earlier quoted context omitted.
A used laboratory grade NGS system can be had for less than 10K https://www.ebay.com/itm/265148387179 Nanopore is still not quite ready yet for precise and high accuracy sequencing. Give it another five years.
Interested outsider here; I work with a lot of HCLS research customers but don't have a biology-related background. Can you explain the problems with the Nanopore sequencer accuracy in more detail? Basically, I was wondering if I could get one for myself and sequence my own genome, then user the data to learn about life-sciences computing techniques. If I were to buy one of the USB-attachable devices and run it, is t…
https://genomebiology.biomedcentral.com/articles/10.1186/s13...
I guess there are limits to ensemble methods if the underlying accuracy doesn't increase. I don't work on gene sequencing algorithms but from what I understand of ML ensemble techniques, there are certain assumptions regarding the underlying independence of the errors. The errors for nanopore should be uniform but I am not sure. Any molecular biologist here care to comment?