How to sequence your own DNA at home
bradleywoolf.com
How to sequence your own DNA at home
1–10 of 161 posts
Re: How to sequence your own DNA at home
#2I wish this had some discussion of the results. The earlier reports about this sensor and process were very mixed. It’s a cool process either way, but I’d like to know how usable the real world output can be.
Re: How to sequence your own DNA at home
#3What is the accuracy in this ? Aka if I run the experiment 10 times how many differences will i get? I don’t have a physical sense on what would be a good number.
Re: How to sequence your own DNA at home
#4https://www.the-odin.com/whole-genome-sequencing-30x/
If you want it quick and cheap(er) - 599.00
Re: How to sequence your own DNA at home
#5> This is intended to be read by AI
Fuck this
Re: How to sequence your own DNA at home
#6What is the accuracy in this ? Aka if I run the experiment 10 times how many differences will i get? I don’t have a physical sense on what would be a good number.
You would get a lot of differences, but the errors would cancel each other out with enough depth of coverage.
This technology's baseline accuracy is around 95% per base, so 10x reads of every segment in the sample would give >99% accuracy for each base after aligning the reads with each other.
Re: How to sequence your own DNA at home
#7I am very impressed with the, why wait? just do it now approach to the future.
which while not here, IS there.
Re: How to sequence your own DNA at home
#8https://www.the-odin.com/whole-genome-sequencing-30x/ If you want it quick and cheap(er) - 599.00
If it's an US-based lab, aren't they subject to CLIA with all its retention requirements?
For $7.5k+ you get a guaranteed privacy (as other comments suggest, other properties may vary, but at least the data never leaves your home).
Re: How to sequence your own DNA at home
#9What is the accuracy in this ? Aka if I run the experiment 10 times how many differences will i get? I don’t have a physical sense on what would be a good number.
Oxford Nanopore unfortunately has a high error rate (3-5%) compared to other sequencing technologies. And the errors are non-random
Re: How to sequence your own DNA at home
#10What is the accuracy in this ? Aka if I run the experiment 10 times how many differences will i get? I don’t have a physical sense on what would be a good number.
You would get a lot of differences, but the errors would cancel each other out with enough depth of coverage. This technology's baseline accuracy is around 95% per base, so 10x reads of every segment in the sample would give >99% accuracy for each base after aligning the reads with each other. https://en.wikipedia.org/wiki/Coverage_(genetics)
> so 10x reads of every segment in the sample would give >99% accuracy for each base after aligning the reads with each other
This assumes random errors, which IIRC isn't the case for Oxford Nanopore.