Viewing profile — nebfield
nebfield
HN member- Joined
- Mon, Jun 27, 2022, 7:45 AM UTC
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About nebfield
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Recent public activity
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Comment #31945386
Very small amounts are sampled, on the order of grams. I'm not sure exactly, I worked on the bioinformatics side of things. I think an Illumina MiSeq requires 50 - 500 nanograms of…
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Comment #31939496
The burden of proof for a coeliac disease diagnosis is quite high: a biopsy from an endoscopy that shows classic signs of damaged villi (from autoimmune mechanisms), or a blood tes…
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Comment #31939344
The paper uses 16S rDNA sequencing, which is a bit old fashioned now but it was a good method when the paper was published. The steps basically involve: 1. Extract all DNA from poo…
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Comment #31891710
Were you tested for coeliac disease? Diagnosis can be difficult, and the standard blood tests don't always pick it up. It might be a good idea to avoid wheat, barley, rye, and oats…