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koeng

HN member
Joined
Fri, Feb 22, 2019, 9:31 PM UTC
HN karma
1,837
Public activity
423 items

About koeng

It'd be pretty cool to build a cell, wouldn't it?

keonigandall.com

synbio25.com

koeng101 X gmail.com

github.com/koeng101/dnadesign

Recent public activity

  1. story
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    Comment #48087148

    Another thing I’m working on: homemade linen. Right now I just germinated a 4x8 bed with flax for fiber. The plan is to grow it for 100 days or so and then harvest, dry, ret, dry, …

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    Comment #48087134

    I’m smelting ore! I got into creating my own rings, and I’d really like to create one with ore I harvest myself. Gold is too hard and silver can be kinda dangerous, but malachite i…

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    Comment #47957303

    I met Craig about a year ago or so at a synthetic biology conference. Even though his institute was the one which created the first synthetic cell, he pretty much just talked about…

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    Comment #47693343

    I do some synthetic biology as a hobby - genetically engineered a baker’s yeast to produce grape aroma and then baked bread with it, and gave it to like 100 people at an event I wa…

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    Comment #46947861

    if 10x the average amount of microplastics are showing changes that are approximately equivalent to hormonal or behavioral changes, it's not a significant factor to be worried abou…

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    Comment #46947811

    eh, not that concerned

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    Comment #46939724

    Microplastics are bad. People are concerned that there are microplastics in your balls! And that this could epigenetically affect downstream generations. I want to test that theory…

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    Comment #46917901

    I work in DNA assembly and synthesis. Here is my take: They don't use oligo pools - "This capacity may be adapted to use large oligo pools to substantially reduce the cost per cons…

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    Comment #46906070

    I am also a fan of SQLite. One of the best parts during development is how easy it is to spin up and spin down databases for full integration tests without containers or anything. …

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    Comment #46620899

    I don’t think it’s legal for me to sell it (it is a genetically modified organism used in food), but I’d like to figure out if I can just give it away

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    Comment #46620885

    keonigandall.com

  14. comment
    Comment #46598563

    Very neat! How do you standardize the gluten free mixes? I’ve been thinking about trying this since my mom is gluten free

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    Comment #46598546

    Already answered there: I’m using bakers yeast, not lab yeast (store-bought S cerevisiae). It’s not haploid, often it’s tetraploid. HR doesn’t guarantee homozygous transformation. …

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    Comment #46598521

    I’d recommend buying an Odin kit and just trying it. Doesn’t take THAT much to get into genetic engineering. The tough part is mostly the finesse in the simple things, like trying …

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    Comment #46598503

    Eh, other than the electroporator I could probably do it for about $100-$200 bucks of equipment if I had a decent kitchen. Reagents probably about $300, but you can use em in a bun…

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    Comment #46598476

    I’ve only done grape so far. It’s on the verge of subtle vs unsubtle. If you’re real used to smelling yeast OR are a woman who has a strong sense of smell, you can smell it. Otherw…

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    Comment #46578654

    I really need to do a write-up. I kinda just whip up the easiest path and do it. For example for the grape, I needed to knock out some tryptophan synthesis genes so I could redirec…

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    Comment #46578515

    I’m genetically engineering yeasts to make subtly flavored breads. I’ve already done grape aroma, now working on wintergreen. Also working on a red chamomile (using beat red biosyn…

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    Comment #46240074

    Neat! Would this mainly be used for JavaScript servers running bun (ie, not end users)?

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    Comment #46207419

    “The Panopticon is good”

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    Comment #45870004

    I've been working on a sillier project lately. Green teeth! Lumina has made a probiotic strain that is able to, theoretically, prevent cavities. I don't care that much about, but I…

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    Comment #45855207

    I don’t think Java and Rust were so ok with completely removing features. For example, in Zig 0.15 they completely overhauled the io, meaning all libraries now have to rewrite up u…

  25. comment
    Comment #45735675

    95% for humans. Who failed to get the butter?