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billiontoone

HN member
Joined
Tue, Oct 13, 2015, 5:45 PM UTC
HN karma
99
Public activity
10 items

About billiontoone

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Recent public activity

  1. comment
    Comment #22809351

    Thanks; that is a good question. In our laboratory, we have a ratio of 10:1 for PCR to qPCR instruments. In the new laboratory that we are constructing, the ratio is 50:1. It was s…

  2. comment
    Comment #22809049

    $15 is half the price of even the bare minimum qPCR kits (e.g., TaqPath). We need to buy the reagents from NEB, IDT, and others and work with a contract manufacturer to mix it into…

  3. comment
    Comment #22808941

    Thanks for the question. Last week, I sent half-a-page of instructions in a hastily written e-mail to a scientist working for the Switzerland government response team, and they tol…

  4. comment
    Comment #22808901

    I think we can scale it to significantly more than 1M a day, but it would require the relaxation of certain regulations that currently prevent collaboration between clinical labora…

  5. comment
    Comment #22808859

    Roche 6600/8800 instruments are state-of-the-art automated RT-qPCR machines. We need them as well as other COVID-19 testing instruments. That said, it is easier to ship the test ki…

  6. comment
    Comment #22808726

    We have the data both with extraction and without and show that it does not make a difference with qSanger. In Figure 4, we add VTM directly to PCR reactions. Seracare VTM samples …

  7. comment
    Comment #22808674

    Both the methods and David's twitter clearly describe the linear regression as the method and how it works with spike-in. In practice, it is a lot more complicated, as the signal-t…

  8. comment
    Comment #22808652

    If it is specific to COVID-19 testing, we will not seek anything, as long as the end-user is not financially benefiting from it or importantly, selling qSanger kits. If they need o…

  9. comment
    Comment #22808539

    I don't think we are in any way undervaluing the serological tests. A great serological test would be very useful. However, because of the indirect measurement, they tend not to be…

  10. comment
    Comment #22808448

    CEO of BillionToOne here. We have already generated and put into our scientific manuscript the data that FDA requires for EUA. As a high-complexity CLIA-licensed clinical laborator…